Porcine Squamous Cell Carcinoma Antigen 2 ELISA Kit | SCCA2 elisa kit
Porcine Squamous Cell Carcinoma Antigen 2 ELISA Kit
Reactivity
Porcine
Synonyms
Squamous Cell Carcinoma Antigen 2; Porcine Squamous Cell Carcinoma Antigen 2 ELISA Kit; SCCA2 elisa kit
Reactivity
Porcine
Specificity
This assay has high sensitivity and excellent specificity for detection of SCCA2. No significant cross-reactivity or interference between SCCA2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SCCA2 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SCCA2 elisa kit
Intended Uses: This SCCA2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine SCCA2. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: SCCA2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SCCA2 antibody and an SCCA2-HRP conjugate. The assay sample and buffer are incubated together with SCCA2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SCCA2 concentration since SCCA2 from samples and SCCA2-HRP conjugate compete for the anti-SCCA2 antibody binding site. Since the number of sites is limited, as more sites are occupied by SCCA2 from the sample, fewer sites are left to bind SCCA2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SCCA2 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: SCCA2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SCCA2 antibody and an SCCA2-HRP conjugate. The assay sample and buffer are incubated together with SCCA2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SCCA2 concentration since SCCA2 from samples and SCCA2-HRP conjugate compete for the anti-SCCA2 antibody binding site. Since the number of sites is limited, as more sites are occupied by SCCA2 from the sample, fewer sites are left to bind SCCA2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SCCA2 concentration in each sample is interpolated from this standard curve.
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