Mouse Thrombospondin 2 (THBS2) ELISA Kit | THBS2 elisa kit

Mouse Thrombospondin 2 (THBS2) ELISA Kit

Cat. Num. AAA2023461

• Gene Names: THBS2; TSP2
• Reactivity: Mouse
• Synonyms: Thrombospondin 2 (THBS2); Mouse Thrombospondin 2 (THBS2) ELISA Kit; TSP2; THBS2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Thrombospondin 2 (THBS2).; No significant cross-reactivity or interference between Thrombospondin 2 (THBS2) and analogues was observed.
• Sequence Length: 1,172

• Assay Type: Double-antibody Sandwich
• Samples: Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.135ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thrombospondin 2 (THBS2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thrombospondin 2 (THBS2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for THBS2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thrombospondin 2 (THBS2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thrombospondin 2 (THBS2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thrombospondin 2 (THBS2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thrombospondin 2 (THBS2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for THBS2 elisa kit

Hematology

NCBI and Uniprot Product Information

• NCBI GI #: 307506
• NCBI GeneID: 7058
• UniProt Accession #: P35442; Q5RI52; A6H8N1; A7E232
• Molecular Weight: 129,991 Da
• NCBI Official Full Name: thrombospondin 2
• NCBI Official Synonym Full Names: thrombospondin 2
• NCBI Official Symbol: THBS2
• NCBI Official Synonym Symbols: TSP2
• NCBI Protein Information: thrombospondin-2
• UniProt Protein Name: Thrombospondin-2
• Protein Family: Thrombospondin
• UniProt Gene Name: THBS2
• UniProt Synonym Gene Names: TSP2
• UniProt Entry Name: TSP2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration. [provided by RefSeq, Jul 2008]

Uniprot Description

THBS2: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties. Genetic variations in THBS2 may be a cause of susceptibility to intervertebral disk disease (IDD); also known as lumbar disk herniation (LDH). IDD is one of the most common musculo-skeletal disorders and the predominant cause of low-back pain and unilateral leg pain. Belongs to the thrombospondin family.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6q27

Cellular Component: extracellular region; basement membrane

Molecular Function: heparin binding; protein binding; calcium ion binding

Biological Process: positive regulation of synaptogenesis; negative regulation of angiogenesis; cell adhesion

Disease: Intervertebral Disc Disease