Goat anti-Human Cathepsin B Antibody | anti-CTSB antibody

Goat Anti-Cathepsin B Antibody

Cat. Num. AAA423870

• Gene Names: CTSB; APPS; CPSB; RECEUP
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: Cathepsin B; Goat Anti-Cathepsin B Antibody; CTSB; cathepsin B; APPS; CPSB; APP secretase; amyloid precursor protein secretase; cathepsin B1; cysteine protease; anti-CTSB antibody

• Host: Goat
• Reactivity: Human
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Sequence Length: 339

• Applicable Applications for anti-CTSB antibody: Peptide ELISA (EIA), WB, Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: Peptide ELISA: antibody detection limit dilution 1:64000; WB: Approx 35kDa band observed in lysates of cell line HepG2 and in Human Lung, Human Colo-rectal carcinoma and Human Brain Glio-Blastoma cancer lysates (calculated MW of 37.8xkDa according to NP_001899.1). Recommended concentration: 0.5-2ug/ml. Primary incubation 1 hour at room temperature.; IF: Strong expression of the protein seen in the cytoplasm/ vesicles of HeLa cells. Recommended concentration: 10ug/ml.; FC/FACS: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Immunogen: Peptide with sequence SDRICIHTNAHVSVE, from the internal region of the protein sequence according to NP_001899.1.
• Peptide Sequence: SDRICIHTNAHVSVE
• Epitope: Internal region
• Important comments: Reported variants represent identical protein: NP_680091.1, NP_680090.1, NP_680092.1, NP_001899.1, NP_680093.1.
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of HepG2 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)

Western Blot (WB)

((1ug/ml) staining of Human Lung (A) and HCORC (B) and (0.5ug/ml) HCBGLC (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)

Immunofluorescence (IF)

(Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/vesicle and extracellular staining. Th)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol)

NCBI and Uniprot Product Information

• NCBI GI #: 4503139
• NCBI GeneID: 1508
• NCBI Accession #: NP_001899.1
• NCBI GenBank Nucleotide #: NP_001899.1
• UniProt Accession #: P07858
• NCBI Official Full Name: cathepsin B isoform 1 preproprotein
• NCBI Official Synonym Full Names: cathepsin B
• NCBI Official Symbol: CTSB
• NCBI Official Synonym Symbols: APPS; CPSB; RECEUP
• NCBI Protein Information: cathepsin B
• UniProt Protein Name: Cathepsin B
• Protein Family: Cathepsin
• UniProt Gene Name: CTSB
• UniProt Synonym Gene Names: CPSB; APPS
• UniProt Entry Name: CATB_HUMAN

NCBI Description

This gene encodes a member of the C1 family of peptidases. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin B light and heavy chains, which can dimerize to form the double chain form of the enzyme. This enzyme is a lysosomal cysteine protease with both endopeptidase and exopeptidase activity that may play a role in protein turnover. It is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP has been suggested to be a causative factor in Alzheimer's disease, the most common cause of dementia. Overexpression of the encoded protein has been associated with esophageal adenocarcinoma and other tumors. Multiple pseudogenes of this gene have been identified. [provided by RefSeq, Nov 2015]

Uniprot Description

CTSB: Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. Dimer of a heavy chain and a light chain cross-linked by a disulfide bond. Interacts with SRPX2. Belongs to the peptidase C1 family.

Protein type: Autophagy; Motility/polarity/chemotaxis; EC 3.4.22.1; Protease

Chromosomal Location of Human Ortholog: 8p22

Cellular Component: extracellular space; mitochondrion; intracellular membrane-bound organelle; perinuclear region of cytoplasm; lysosome; nucleolus; extracellular region; melanosome; intracellular

Molecular Function: collagen binding; peptidase activity; proteoglycan binding; protein binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: regulation of apoptosis; extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; epithelial cell differentiation; proteolysis involved in cellular protein catabolic process; regulation of catalytic activity; toll-like receptor signaling pathway; innate immune response; decidualization; proteolysis