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Western Blot (WB) (The anti-GKP2 Pab is used in Western blot to detect GKP2 in Jurkat tissue lysate)

Rabbit anti-Human Glycerol Kinase 2 Polyclonal Antibody | anti-GK2 antibody

Glycerol Kinase 2, CT (Glycerol Kinase Testis Specific 2, Glycerokinase 2, GK 2, GK2, ATP:glycerol 3-phosphotransferase, GKP2, GKTA) (APC)

Gene Names
GK2; GKP2; GKTA
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
Glycerol Kinase 2; Polyclonal Antibody; CT (Glycerol Kinase Testis Specific 2; Glycerokinase 2; GK 2; GK2; ATP:glycerol 3-phosphotransferase; GKP2; GKTA) (APC); EC=2.7.1.30; anti-GK2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human GPK2.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-GK2 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:50-1:100
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human GPK2 (KLH).
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-GKP2 Pab is used in Western blot to detect GKP2 in Jurkat tissue lysate)

Western Blot (WB) (The anti-GKP2 Pab is used in Western blot to detect GKP2 in Jurkat tissue lysate)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Product Categories/Family for anti-GK2 antibody
References
1. Sargent, C.A., et al., Hum. Mol. Genet. 3(8):1317-1324 (1994).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
60,594 Da
NCBI Official Full Name
glycerol kinase 2
NCBI Official Synonym Full Names
glycerol kinase 2
NCBI Official Symbol
GK2
NCBI Official Synonym Symbols
GKP2; GKTA
NCBI Protein Information
glycerol kinase 2; ATP:glycerol 3-phosphotransferase 2; glycerokinase 2; glycerol kinase pseudogene 2; glycerol kinase testis specific 2; glycerol kinase, testis specific 2
UniProt Protein Name
Glycerol kinase 2
Protein Family
UniProt Gene Name
GK2
UniProt Synonym Gene Names
GKP2; GKTA; GK 2
UniProt Entry Name
GLPK2_HUMAN

Uniprot Description

GK2: Key enzyme in the regulation of glycerol uptake and metabolism. Belongs to the FGGY kinase family.

Protein type: EC 2.7.1.30; Lipid Metabolism - glycerolipid; Kinase, other

Chromosomal Location of Human Ortholog: 4q13

Cellular Component: mitochondrial outer membrane

Molecular Function: glycerol kinase activity; ATP binding

Biological Process: glycerol-3-phosphate metabolic process; phosphorylation; glycerol catabolic process

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Product Notes

The GK2 gk2 (Catalog #AAA6478430) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Glycerol Kinase 2, CT (Glycerol Kinase Testis Specific 2, Glycerokinase 2, GK 2, GK2, ATP:glycerol 3-phosphotransferase, GKP2, GKTA) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Glycerol Kinase 2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-1:100 FLISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GK2 gk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glycerol Kinase 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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