A53T Mutant Alpha Synuclein Active Protein | A53T active protein

Active Human Recombinant A53T Mutant Alpha Synuclein Protein Preformed Fibrils (Type 1)

Cat. Num. AAA8005532

• Gene Names: SNCA; PD1; NACP; PARK1; PARK4
• Applications: Western Blot, SDS-Page
• Purity: >95% pure using SDS-PAGE analysis.; Ion-Exchange Purified
• Synonyms: A53T Mutant Alpha Synuclein; Active Human Recombinant A53T Mutant Alpha Synuclein Protein Preformed Fibrils (Type 1); Alpha Synuclein Protein; A53T mutant alpha synuclein; A53T mutated SNCA; A53T Alpha synuclein PFFs; Ala-53-->thr mutant alpha synuclein; Alpha synuclein pre-formed fibrils; Alpha synuclein aggregates; Alpha synuclein protein aggregates; Active Alpha synuclein aggregates; Alpha-synuclein protein; Non-A beta component of AD amyloid protein; Non-A4 component of amyloid precursor protein; NACP protein; SNCA protein; PARK1 protein; Alpha synuclein monomers; SYN protein; Parkinson disease familial 1 Protein; A53T active protein

• Host: E Coli
• Specificity: ~14.46 kDa
• Purity/Purification: >95% pure using SDS-PAGE analysis.; Ion-Exchange Purified
• Form/Format: PBS pH 7.4
• Concentration: 2 mg/mL (varies by lot)
• Sequence Length: Full Length

• Applicable Applications for A53T active protein: Western Blot (WB), SDS-PAGE, In Vitro Assay
• Species: Human
• Biological Activity: Endogenous alpha-synuclein phosphorylation. 100 uM A53T alpha synuclein protein monomer seeded with 10 nM A53T alpha synuclein protein PFF in 25 uM Thioflavin T (PBS pH 7.4, 100 ul reaction volume) generated a fluorescence intensity of 28 000 Relative Fluorescence Units after incubation at 37 degree C with shaking at 600 rpm for 56 hours. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.
• Cellular Localization: Cytoplasm; Membrane; Nucleus
• Protein Length: Full Length
• Preparation and Storage: Store at-80 degree C.

Testing Data

(TEM of A53T alpha synuclein preformed fibrils (SPR-326))

Testing Data

(Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show a limited increase in fluorescence (correlated to alpha synuclein aggregation) over time in A53T alpha synuclein monomers (SPR-325). A much greater increase in fluorescence is seen when 100 uM monomer (SPR-325) is combined with 10 nM of fibrils (SPR-326) as the fibrils seed the formation of new fibrils from the pool of active monomers. Thioflavin T ex = 450 nm, em = 485 nm.)

Related Product Information for A53T active protein

Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions; inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6). The A53T mutation is a missense point mutation where alanine is replaced by threonine at the 53rd amino acid. This mutation has been linked to early-onset Parkinson's Disease (7) and increased rates of alpha synuclein fibrillization (8).

Product Categories/Family for A53T active protein

Neuroscience; Neurodegeneration; Alzheimer's Disease; Tangles & Tau; Neuroscience; Neurodegeneration; Parkinson's Disease; Synuclein

References

1. "Genetics Home Reference: SNCA". US National Library of Medicine. (2013). 2. Zhang L., et al. (2008) Brain Res. 1244: 40-52. 3. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117. 4. Kokhan V.S., Afanasyeva M.A., Van'kin G. (2012) Behav. Brain. Res. 231(1): 226-230. 5. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840. 6. Mezey E., et al. (1998) Nat Med. 4(7): 755-757. 7. Polymeropoulos, M. H. (1998). Science. 276(5321), 2045-2047. 8. Conway, K.E., et al. (1998). Nat Med. 4(11):1318-20.

NCBI and Uniprot Product Information

• NCBI GI #: 4507109
• NCBI GeneID: 6622
• NCBI Accession #: NP_000336.1
• NCBI GenBank Nucleotide #: NP_000336.1
• UniProt Accession #: P37840 ; P37840
• NCBI Official Full Name: alpha-synuclein isoform NACP140
• NCBI Official Synonym Full Names: synuclein alpha
• NCBI Official Symbol: SNCA
• NCBI Official Synonym Symbols: PD1; NACP; PARK1; PARK4
• NCBI Protein Information: alpha-synuclein

NCBI Description

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2016]