Glycine N-Methyltransferase Recombinant Protein | GNMT recombinant protein

Recombinant Human Glycine N-Methyltransferase

Cat. Num. AAA143914

• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Synonyms: Glycine N-Methyltransferase; Recombinant Human Glycine N-Methyltransferase; GNMT Human; Glycine N-methyltransferase Human Recombinant; Glycine N-methyltransferase; GNMT; GNMT recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by SDS-PAGE.
• Form/Format: The GNMT solution contains 20mM Tris pH 8.0 & 20% glycerol.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MVDSVYRTRS LGVAAEGLPD QYADGEAARV WQLYIGDTRS RTAEYKAWLL GLLRQHGCQR VLDVACGTGV DSIMLVEEGF SVTSVDASDK MLKYALKERW NRRHEPAFDK WVIEEANWMT LDKDVPQSAE GGFDAVICLG NSFAHLPDCK GDQSEHRLAL KNIASMVRAG GLLVIDHRNY DHILSTGCAP PGKNIYYKSD LTKDVTTSVL IVNNKAHMVT LDYTVQVPGA GQDGSPGLSK FRLSYYPHCL ASFTELLQAA FGGKCQHSVL GDFKPYKPGQ TYIPCYFIHV LKRTD
• Sequence Length: 295

• Preparation and Storage: GNMT although stable 4 degree C for 4 weeks, should be stored desiccated below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for GNMT recombinant protein

Description: GNMT Human Recombinant fused with 20 amino acid His-Tag tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing a total of 315 amino acids (1-295 a.a.) and having a molecular mass of 34.9 kDa.The GNMT is purified by proprietary chromatographic techniques.

Introduction: GNMT is an enzyme that catalyzes the conversion of S-adenosyl-L-methionine with glycine to S-adenosyl-L-homocysteine and sarcosine. GNMT is located in the cytoplasm and acts as a homotetramer. Defects in the GNMT gene causes of GNMT deficiency (hypermethioninemia). GNMT affects DNA methylation by regulating the ratio of S-adenosylmethionine to S-adenosylhomocystine and is involved in the detoxification pathway in liver cells. GNMT expression is diminished in human hepatocellular carcinoma (HCC). GNMT catalyzes the methylation of glycine by using s- adenosylmethionine (adomet) to form n-methylglycine (sarcosine) with the concomitant production of s-adenosylhomocysteine (adohcy). GNMT plays an essential role in the regulation of tissue concentration of adomet and of metabolism of methionine.

Product Categories/Family for GNMT recombinant protein

Enzymes; Transferase

NCBI and Uniprot Product Information

• NCBI GI #: 9506741
• NCBI GeneID: 27232
• NCBI Accession #: NP_061833.1
• NCBI GenBank Nucleotide #: NM_018960.4
• UniProt Accession #: Q14749; Q5T8W2; Q9NNZ1; Q9NS24
• Molecular Weight: 32,742 Da
• NCBI Official Full Name: glycine N-methyltransferase
• NCBI Official Synonym Full Names: glycine N-methyltransferase
• NCBI Official Symbol: GNMT
• NCBI Protein Information: glycine N-methyltransferase; HEL-S-182mP; epididymis secretory sperm binding protein Li 182mP
• UniProt Protein Name: Glycine N-methyltransferase
• Protein Family: Glycine N-methyltransferase
• UniProt Gene Name: GNMT
• UniProt Entry Name: GNMT_HUMAN

NCBI Description

The protein encoded by this gene is an enzyme that catalyzes the conversion of S-adenosyl-L-methionine (along with glycine) to S-adenosyl-L-homocysteine and sarcosine. The encoded protein is found in the cytoplasm and acts as a homotetramer. Defects in this gene are a cause of GNMT deficiency (hypermethioninemia). [provided by RefSeq, Oct 2008]

Uniprot Description

GNMT: Catalyzes the methylation of glycine by using S- adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine. Defects in GNMT are the cause of glycine N- methyltransferase deficiency (GNMT deficiency); also known as hypermethioninemia. The only clinical abnormalities in patients with this deficiency are mild hepatomegaly and chronic elevation of serum transaminases. Belongs to the class I-like SAM-binding methyltransferase superfamily. Glycine N-methyltransferase family.

Protein type: Amino Acid Metabolism - glycine, serine and threonine; EC 2.1.1.20; Methyltransferase

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: cytoplasm

Molecular Function: protein binding; glycine N-methyltransferase activity; glycine binding; folic acid binding

Biological Process: glycogen metabolic process; methylation; protein modification process; S-adenosylmethionine metabolic process; methionine metabolic process; regulation of gluconeogenesis; one-carbon compound metabolic process; protein homotetramerization

Disease: Glycine N-methyltransferase Deficiency