Glycine N-methyltransferase Active Protein | GNMT active protein

Glycine N-methyltransferase, Human Recombinant

Cat. Num. AAA849862

• Purity: >95% by SDS-PAGE
• Synonyms: Glycine N-methyltransferase; Human Recombinant; GNMT; GNMT active protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol
• Sequence: MGSSHHHHHHSSGLVPRGSHMVDSVYRTRSLGVAAEGLPDQYADGEAARVWQLYIGDTRSRTAEYKAWLLGLLRQHGCQRVLDVACGTGVDSIMLVEEGFSVTSVDASDKMLKYALKERWNRRHEPAFDKWVIEEANWMTLDKDVPQSAEGGFDAVICLGNSFAHLPDCKGDQSEHRLALKNIASMVRAGGLLVIDHRNYDHILSTGCAPPGKNIYYKSDLTKDVTTSVLIVNNKAHMVTLDYTVQVPGAGQDGSPGLSKFRLSYYPHCLASFTELLQaaFGGKCQHSVLGDFKPYKPGQTYIPCYFIHVLKRTD

• Gene Source: Human
• Endotoxin: <1.0 EU per/ug
• Biological Activity: Specific activity > 100 nmol/min/mg; Measured by its ability to transfer 1.0 nmole of methyl group per minute at 37 degree C.
• Preparation and Storage: Store at -20 degree C; Centrifuge the vial prior to opening.; Shelf life: ~12 months

SDS-Page

Related Product Information for GNMT active protein

Glycine N-methyltransferase, also known as GNMT, catalyzes the synthesis of N-methylglycine (sarcosine) from glycine using S-adenosylmethionine (AdoMet) as the methyl donor. This protein affects DNA methylation by regulating the ratio of S-adenosylmethionine to S-adenosylhomocystine and participates in the detoxification pathway in liver cells. Also it is reported that GNMT expression is diminished in human hepatocellular carcinoma (HCC). Recombinant human GNMT protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques. Regulate tissue concentration of AdoMet and metabolism of methionine

Product Categories/Family for GNMT active protein

Proteins; Enzymes; Proteins & Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 12644416
• NCBI GeneID: 27232
• UniProt Accession #: Q14749; Q5T8W2; Q9NNZ1; Q9NS24
• Molecular Weight: 295
• NCBI Official Full Name: Glycine N-methyltransferase
• NCBI Official Synonym Full Names: glycine N-methyltransferase
• NCBI Official Symbol: GNMT
• NCBI Protein Information: glycine N-methyltransferase; HEL-S-182mP; epididymis secretory sperm binding protein Li 182mP
• UniProt Protein Name: Glycine N-methyltransferase
• Protein Family: Glycine N-methyltransferase
• UniProt Gene Name: GNMT
• UniProt Entry Name: GNMT_HUMAN

NCBI Description

The protein encoded by this gene is an enzyme that catalyzes the conversion of S-adenosyl-L-methionine (along with glycine) to S-adenosyl-L-homocysteine and sarcosine. The encoded protein is found in the cytoplasm and acts as a homotetramer. Defects in this gene are a cause of GNMT deficiency (hypermethioninemia). [provided by RefSeq, Oct 2008]

Uniprot Description

GNMT: Catalyzes the methylation of glycine by using S- adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine. Defects in GNMT are the cause of glycine N- methyltransferase deficiency (GNMT deficiency); also known as hypermethioninemia. The only clinical abnormalities in patients with this deficiency are mild hepatomegaly and chronic elevation of serum transaminases. Belongs to the class I-like SAM-binding methyltransferase superfamily. Glycine N-methyltransferase family.

Protein type: Amino Acid Metabolism - glycine, serine and threonine; Methyltransferase; EC 2.1.1.20

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: cytoplasm

Molecular Function: protein binding; glycine N-methyltransferase activity; glycine binding; folic acid binding

Biological Process: methylation; glycogen metabolic process; S-adenosylmethionine metabolic process; protein modification process; regulation of gluconeogenesis; methionine metabolic process; one-carbon compound metabolic process; protein homotetramerization

Disease: Glycine N-methyltransferase Deficiency