Typical Testing Data/Standard Curve (for reference only)
Human SHP1 ELISA Kit | SHP1 elisa kit
Human SHP1 (Tyrosine-protein phosphatase non-receptor type 6) ELISA Kit
Reactivity
Human
Synonyms
SHP1; Human SHP1 (Tyrosine-protein phosphatase non-receptor type 6) ELISA Kit; SHP1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection ofSHP1. No significant cross-reactivity or interference betweenSHP1and analogues was observed.
Sequence Length
423
Samples
serum, plasma, tissue homogenatesand other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/ml
Sensitivity
<0.094ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high levelSHP1were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high levelSHP1were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store at 4 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SHP1 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- SHP1antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- SHP1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the SHP1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of SHP1 can be calculated.
NCBI and Uniprot Product Information
Uniprot Description
Involved in CDC48-dependent protein degradation through the ubiquitin/proteasome pathway. Direct or indirect positive regulator of GLC7 activity.
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Product Notes
The Human SHP1 shp1 (Catalog #AAA7606994) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7606994 ELISA Kit recognizes Human SHP1. It is sometimes possible for the material contained within the vial of "SHP1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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