Typical Testing Data/Standard Curve (for reference only)
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Monkey Growth Hormone Binding Protein ELISA Kit | GHBP elisa kit
Monkey Growth Hormone Binding Protein ELISA Kit
Reactivity
Monkey
Synonyms
Growth Hormone Binding Protein; Monkey Growth Hormone Binding Protein ELISA Kit; GHBP elisa kit
Reactivity
Monkey
Specificity
This assay has high sensitivity and excellent specificity for detection of GHBP. No significant cross-reactivity or interference between GHBP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GHBP and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for GHBP elisa kit
Intended Uses: This GHBP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey GHBP. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: GHBP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GHBP antibody and an GHBP-HRP conjugate. The assay sample and buffer are incubated together with GHBP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GHBP concentration since GHBP from samples and GHBP-HRP conjugate compete for the anti-GHBP antibody binding site. Since the number of sites is limited, as more sites are occupied by GHBP from the sample, fewer sites are left to bind GHBP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GHBP concentration in each sample is interpolated from this standard curve.
Principle of the Assay: GHBP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GHBP antibody and an GHBP-HRP conjugate. The assay sample and buffer are incubated together with GHBP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GHBP concentration since GHBP from samples and GHBP-HRP conjugate compete for the anti-GHBP antibody binding site. Since the number of sites is limited, as more sites are occupied by GHBP from the sample, fewer sites are left to bind GHBP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GHBP concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,944 Da
NCBI Official Full Name
glucagon
NCBI Official Synonym Full Names
glucagon<
NCBI Official Symbol
GCG
NCBI Protein Information
glucagon
UniProt Protein Name
Glucagon
UniProt Gene Name
GCG
UniProt Synonym Gene Names
GRPP; OXM; OXY; GLP-1; GLP-1(7-37); GLP-1(7-36); GLP-2
UniProt Entry Name
GLUC_BOVIN
Uniprot Description
Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia ().
Research Articles on GHBP
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Product Notes
The Monkey GHBP gcg (Catalog #AAA754263) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA754263 ELISA Kit recognizes Monkey GHBP. It is sometimes possible for the material contained within the vial of "Growth Hormone Binding Protein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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