Human Melanocyte Stimulating Hormone (MSH) ELISA Kit | MSH elisa kit

Human Melanocyte Stimulating Hormone (MSH) ELISA Kit

Cat. Num. AAA264371

• Gene Names: MC1R; CMM5; MSH-R; SHEP2
• Reactivity: Human
• Synonyms: Melanocyte Stimulating Hormone (MSH); Human Melanocyte Stimulating Hormone (MSH) ELISA Kit; MSH elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.
• Sequence Length: 317

• Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant MSH concentration
• Assay Type: Sandwich
• Detection Range: 10 ng/ml-0.156 ng/ml
• Sensitivity: 0.05 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MSH elisa kit

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human MSH monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 51593766
• NCBI GeneID: 4157
• Molecular Weight: 34,706 Da
• NCBI Official Full Name: Melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor)
• NCBI Official Synonym Full Names: melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor)
• NCBI Official Symbol: MC1R
• NCBI Official Synonym Symbols: CMM5; MSH-R; SHEP2
• NCBI Protein Information: melanocyte-stimulating hormone receptor; MC1-R; melanotropin receptor
• UniProt Protein Name: Melanocyte-stimulating hormone receptor
• UniProt Gene Name: MC1R
• UniProt Synonym Gene Names: MSHR; MSH-R; MC1-R
• UniProt Entry Name: MSHR_HUMAN

NCBI Description

This intronless gene encodes the receptor protein for melanocyte-stimulating hormone (MSH). The encoded protein, a seven pass transmembrane G protein coupled receptor, controls melanogenesis. Two types of melanin exist: red pheomelanin and black eumelanin. Gene mutations that lead to a loss in function are associated with increased pheomelanin production, which leads to lighter skin and hair color. Eumelanin is photoprotective but pheomelanin may contribute to UV-induced skin damage by generating free radicals upon UV radiation. Binding of MSH to its receptor activates the receptor and stimulates eumelanin synthesis. This receptor is a major determining factor in sun sensitivity and is a genetic risk factor for melanoma and non-melanoma skin cancer. Over 30 variant alleles have been identified which correlate with skin and hair color, providing evidence that this gene is an important component in determining normal human pigment variation. [provided by RefSeq, Jul 2008]

Uniprot Description

MC1R: Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Genetic variations in MC1R are a cause of susceptibility to cutaneous malignant melanoma type 5 (CMM5). Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding; melanocortin receptor activity; hormone binding; ubiquitin protein ligase binding; melanocyte stimulating hormone receptor activity; peptide receptor activity, G-protein coupled

Biological Process: melanin biosynthetic process; G-protein signaling, coupled to cyclic nucleotide second messenger; positive regulation of protein kinase B signaling cascade; pigmentation; UV protection; multicellular organismal development; positive regulation of cAMP biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; negative regulation of tumor necrosis factor production; sensory perception of pain

Disease: Albinism, Oculocutaneous, Type Ii; Increased Analgesia From Kappa-opioid Receptor Agonist, Female-specific; Melanoma, Cutaneous Malignant, Susceptibility To, 5; Skin/hair/eye Pigmentation, Variation In, 2