Rat Growth Hormone Binding Protein ELISA Kit | GHBP elisa kit

Rat Growth Hormone Binding Protein ELISA Kit

Cat. Num. AAA721432

• Reactivity: Rat
• Synonyms: Growth Hormone Binding Protein; Rat Growth Hormone Binding Protein ELISA Kit; GHBP elisa kit

• Reactivity: Rat

• Assay Type: Sandwich
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GHBP elisa kit

Intended Uses: This GLUCAGON ELISA kit is intended Laboratory for research use only.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of GLUCAGON in the sample, this GLUCAGON ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus GLUCAGON concentration. The concentration of GLUCAGON in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This GLUCAGON enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for GLUCAGON. Standards or samples are then added to the microtiter plate wells and GLUCAGON if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GLUCAGON present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GLUCAGON are added to each well to "sandwich" the GLUCAGON immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain GLUCAGON and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 27806879
• NCBI GeneID: 280802
• NCBI Accession #: NP_776341.1
• NCBI GenBank Nucleotide #: NM_173916.3
• Molecular Weight: 20,944 Da
• NCBI Official Full Name: glucagon
• NCBI Official Synonym Full Names: glucagon<
• NCBI Official Symbol: GCG
• NCBI Protein Information: glucagon
• UniProt Protein Name: Glucagon
• UniProt Gene Name: GCG
• UniProt Synonym Gene Names: GRPP; OXM; OXY; GLP-1; GLP-1(7-37); GLP-1(7-36); GLP-2
• UniProt Entry Name: GLUC_BOVIN

Uniprot Description

Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia ().