Typical Testing Data/Standard Curve (for reference only)
Guinea Pig Growth Hormone Binding Protein ELISA Kit | GHBP elisa kit
Guinea pig Growth Hormone Binding Protein ELISA Kit
Reactivity
Guinea Pig
Synonyms
Growth Hormone Binding Protein; Guinea pig Growth Hormone Binding Protein ELISA Kit; GHBP elisa kit
Reactivity
Guinea Pig
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GHBP elisa kit
For Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Intended Uses: This GC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig GC, This ELISA kit for research use only!
Principle of the Assay: GC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GC antibody and an GC-HRP conjugate. The assay sample and suffer are incubated together with GC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GC concentration since GC from samples and GC-HRP conjugate compete for the anti-GC antibody binding site. Since the number of sites is limited, as more sites are occupied by GC from the sample, fewer sites are left to bind GC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GC concentration in each sample is interpolated from this standard curve.
Intended Uses: This GC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig GC, This ELISA kit for research use only!
Principle of the Assay: GC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GC antibody and an GC-HRP conjugate. The assay sample and suffer are incubated together with GC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GC concentration since GC from samples and GC-HRP conjugate compete for the anti-GC antibody binding site. Since the number of sites is limited, as more sites are occupied by GC from the sample, fewer sites are left to bind GC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GC concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,944 Da
NCBI Official Full Name
glucagon
NCBI Official Synonym Full Names
glucagon
NCBI Official Symbol
GCG
NCBI Protein Information
glucagon
UniProt Protein Name
Glucagon
UniProt Gene Name
GCG
UniProt Synonym Gene Names
GRPP; OXM; OXY; GLP-1; GLP-1(7-37); GLP-1(7-36); GLP-2
UniProt Entry Name
GLUC_BOVIN
Uniprot Description
Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia ().
Research Articles on GHBP
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Product Notes
The Guinea Pig GHBP gcg (Catalog #AAA724918) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA724918 ELISA Kit recognizes Guinea Pig GHBP. It is sometimes possible for the material contained within the vial of "Growth Hormone Binding Protein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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