GFRA3 / GFR-alpha-3 Recombinant Protein | GFRA3 recombinant protein

Recombinant Human GFRA3 / GFR-alpha-3 Protein (His tag)

Cat. Num. AAA2546405

• Gene Names: GFRA3; GDNFR3
• Purity: > 90 % as determined by reducing SDS-PAGE.
• Synonyms: GFRA3 / GFR-alpha-3; Recombinant Human GFRA3 / GFR-alpha-3 Protein (His tag); GDNFR3; GFRA3 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: > 90 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.
• Sequence: Met 1-Trp 382
• Sequence Length: 400

• Application Notes: The recombinant human GFRa3 consists of 362 amino acids and predicts a molecular mass of 40.8 kDa. As a result of glycosylation, the apparent molecular mass of rhGFRa3 is approximately 50 kDa in SDS-PAGE under reducing conditions.
• Species: Human
• Endotoxin: < 1.0 EU per ug as determined by the LAL method.
• Tag: C-His
• Reconstitution: It is recommended that sterile water(500ul) be added to the vial to prepare a stock solution of 0.1mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Storage: Lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; ; Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

SDS-PAGE

Related Product Information for GFRA3 recombinant protein

Glial cell line derived neurotrophic factor (GDNF) Family Receptor Alpha 3 (GFRA3) or GDNFRa3 is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRA3 / GDNFRa3 is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Glial cell linederived neurotrophic factor (GDNF) and neurturin (NTN) are its binding ligand which are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF promotes the formation of a physical complex between GFRA/GDNFRa and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. The RET is a receptor tyrosine kinase representing the signal-transducing molecule of a multisubunit surface receptor complex for the GDNF, in which GFRA / GDNFRa acts as the ligand-binding component. The neurotrophic growth factor artemin binds selectively to GDNF family receptor ?3 (GFRA3 / GDNFRa3), forming a molecular complex with the co-receptor RET which mediates downstream signaling. This signaling pathway has been demonstrated to play an important role in the survival and maintenance of nociceptive sensory neurons and in the development of sympathetic neurons.

NCBI and Uniprot Product Information

• NCBI GI #: 22035694
• NCBI GeneID: 2676
• NCBI Accession #: NP_001487.2
• NCBI GenBank Nucleotide #: NM_001496.3
• UniProt Accession #: O60609; Q6UW20; Q8IUZ2; B2RA36; B4DMY9
• Molecular Weight: Mol_Mass: 40.8 kDa; AP_Mol_Mass: 50 kDa
• NCBI Official Full Name: GDNF family receptor alpha-3 preproprotein
• NCBI Official Synonym Full Names: GDNF family receptor alpha 3
• NCBI Official Symbol: GFRA3
• NCBI Official Synonym Symbols: GDNFR3
• NCBI Protein Information: GDNF family receptor alpha-3
• UniProt Protein Name: GDNF family receptor alpha-3
• Protein Family: GDNF family receptor
• UniProt Gene Name: GFRA3
• UniProt Synonym Gene Names: GDNF receptor alpha-3; GDNFR-alpha-3; GFR-alpha-3

NCBI Description

The protein encoded by this gene is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor and a member of the GDNF receptor family. It forms a signaling receptor complex with RET tyrosine kinase receptor and binds the ligand, artemin. [provided by RefSeq, Jul 2008]

Uniprot Description

GFRA3: Receptor for the glial cell line-derived neurotrophic factor, ARTN (artemin). Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase. Belongs to the GDNFR family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 5q31.2

Cellular Component: cytoplasm; extrinsic to membrane; intracellular membrane-bound organelle; plasma membrane

Molecular Function: Ras guanyl-nucleotide exchange factor activity; receptor binding

Biological Process: MAPKKK cascade; nervous system development; peripheral nervous system development; signal transduction