Bone Morphogenetic Protein-3b Recombinant Protein | BMP-3 recombinant protein

Human Bone Morphogenetic Protein-3b Recombinant

Cat. Num. AAA553054

• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: Bone Morphogenetic Protein-3b; Human Bone Morphogenetic Protein-3b Recombinant; Human Bone Morphogenetic Protein-3 Recombinant; bmp3; bmp-3; Bone Morphogenetic Protein; BMP-3 recombinant protein

• Host: E Coli
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant BMP-3b was lyophilized from a 0.2 um filtered 1mg/ml 10 mM sodium citrate (pH 3.5) containing 10% glycerol.
• Sequence: MAHVPARTSP GPGPQLLLLL LPLFLLLLRD VAGSHRAPAW SALPAAADGL QGDRDLQRHP GDAAATLGPS AQDMVAVHMH RLYEKYSRQG ARPGGGNTVR SFRARLEVVD QKAVYFFNLT SMQDSEMILT ATFHFYSEPP RWPRALEVLC KPRAKNASGR PLPLGPPTRQ HLLFRSLSQN TATQGLLRGA MALAPPPRGL WQAKDISPIV KAARRDGELL LSAQLDSEER DPGVPRPSPY APYILVYAND LAISEPNSVA VTLQRYDPFP AGDPEPRAAP NNSADPRVRR AAQATGPLQD NELPGLDERP PRAHAQHFHK HQLWPSPFRA LKPRPGRKDR RKKGQEVFMA ASQVLDFDEK TMQKARRKQW DEPRVCSRRY LKVDFADIGW NEWIISPKSF DAYYCAGACE FPMPKIVRPS NHATIQSIVR AVGIIPGIPE PCCVPDKMNS LGVLFLDENR NVVLKVYPNM SVDTCACR
• Sequence Length: 478

• Domain: TGFB
• Host Note: Optimized DNA sequence encoding Human Bone Morphogenetic Protein-3b mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Native human Bone Morphogenetic Protein-3b is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 13 kDa.; ; Recombinant BMP-3b is a disulfide-linked monomer protein consisting of 111 amino acid residue subunits. BMP3 migrates as an approximately 12-13 kDa protein under reducing conditions in SDS-PAGE.
• Preparation and Storage: Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months.; ; Avoid repeated freeze/thaw cycles.

Related Product Information for BMP-3 recombinant protein

BMPs are proteins that act to induce the differentiation of mesenchymal-type cells into chondrocytes and osteoblasts before initiating bone formation. They promote the differentiation of cartilage-forming cells and bone-forming cells near sites of fractures but also at ectopic locations. Some of the proteins induce the synthesis of alkaline phosphatase and collagen in osteoblasts. Some BMPs act directly on osteoblasts and promote their maturation while at the same time suppressing myogenous differentiation. Other BMPs promote the conversion of typical fibroblasts into chondrocytes and are capable also of inducing the expression of an osteoblast phenotype in non-osteogenic cell types. Intracellular signaling following engagement of receptors for some BMP proteins has been shown to involve the action of SMAD proteins.

Product Categories/Family for BMP-3 recombinant protein

Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 4826740
• NCBI GeneID: 2662
• NCBI Accession #: NP_004953.1
• NCBI GenBank Nucleotide #: NM_004962.3
• UniProt Accession #: P55107; Q5VSQ8; Q9UCX6; Q8N6T2
• Molecular Weight: 53,122 Da
• NCBI Official Full Name: bone morphogenetic protein 3B
• UniProt Protein Name: Bone morphogenetic protein 3B
• UniProt Gene Name: GDF10
• UniProt Synonym Gene Names: BMP3B; BMP-3B; BIP; GDF-10
• UniProt Entry Name: BMP3B_HUMAN

NCBI Description

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice suggest that the protein encoded by this gene plays a role in skeletal morphogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

GDF10: is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice suggest that plays a role in skeletal morphogenesis. [provided by RefSeq, Jul 2008]

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q11.22

Cellular Component: extracellular space

Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: regulation of apoptosis; fat cell differentiation; transforming growth factor beta receptor signaling pathway; regulation of MAPKKK cascade; skeletal development; cell development; growth