Rabbit anti-Human, Mouse TCF3 Polyclonal Antibody | anti-TCF3 antibody

TCF3, CT (TCF3, BHLHB21, E2A, ITF1, Transcription factor E2-alpha, Class B basic helix-loop-helix protein 21, Immunoglobulin enhancer-binding factor E12/E47, Immunoglobulin transcription factor 1, Kappa-E2-binding factor, Transcription factor 3, Transcrip

Cat. Num. AAA6354493

• Gene Names: TCF3; E2A; E47; AGM8; ITF1; VDIR; TCF-3; bHLHb21
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: TCF3; Polyclonal Antibody; CT (TCF3; BHLHB21; E2A; ITF1; Transcription factor E2-alpha; Class B basic helix-loop-helix protein 21; Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; Kappa-E2-binding factor; Transcription factor 3; Transcrip; Transcription factor ITF-1; anti-TCF3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TCF3. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-TCF3 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 506-534 in the C-terminal region of human TCF3.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in human uterine tumor tissue lysates (35ug/lane) usingMBS6013650. This demonstrates that MBS6013650 detected the TCF3 protein (arrow).)

Western Blot (WB)

(Western Blot analysis in mouse cerebellum tissue lysates (35ug/lane) usingMBS6013650. This demonstrates that MBS6013650 detected the TCF3 protein (arrow).)

Western Blot (WB)

(Western Blot using MBS6013650 (1:1000) Lane 1: Jurkat whole cell lysate Lane 2: Ramos whole cell lysate Lysates/proteins at 20ug/lane. Secondary IgG (HRP) Goat Anti-Rabbit IgG, (H+L), at 1:10,000. Predicted band size: 68kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue using MBS6013650 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6013650 for immunohistochemistry.)

Product Categories/Family for anti-TCF3 antibody

Antibodies; Transcription Factors

References

1. Hirose, K., et al. Blood 116(6):962-970(2010). 2. Pan, F., et al. Immunogenetics 62(4):237-251(2010). 3. Hauser, J., et al. Mol. Immunol. 47(5):1031-1038(2010). 4. Rittie, L., et al. Aging Cell 8(6):738-751(2009). 5. Pedraza, N., et al. J. Biol. Chem. 284(47):32980-32988(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 27777636
• NCBI GeneID: 6929
• NCBI Accession #: NP_003191.1; NP_001129611.1
• NCBI GenBank Nucleotide #: NM_003200.3
• UniProt Accession #: P15923; P15883; Q14208; Q14635; Q14636; Q2TB39; Q2TB40; Q9UPI9
• Molecular Weight: 68,510 Da
• NCBI Official Full Name: transcription factor E2-alpha isoform E12
• NCBI Official Synonym Full Names: transcription factor 3
• NCBI Official Symbol: TCF3
• NCBI Official Synonym Symbols: E2A; E47; AGM8; ITF1; VDIR; TCF-3; bHLHb21
• NCBI Protein Information: transcription factor E2-alpha
• UniProt Protein Name: Transcription factor E2-alpha
• Protein Family: TCF3 fusion partner
• UniProt Gene Name: TCF3
• UniProt Synonym Gene Names: BHLHB21; E2A; ITF1; bHLHb21; TCF-3
• UniProt Entry Name: TFE2_HUMAN

NCBI Description

This gene encodes a member of the E protein (class I) family of helix-loop-helix transcription factors. E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. E proteins play a critical role in lymphopoiesis, and the encoded protein is required for B and T lymphocyte development. Deletion of this gene or diminished activity of the encoded protein may play a role in lymphoid malignancies. This gene is also involved in several chromosomal translocations that are associated with lymphoid malignancies including pre-B-cell acute lymphoblastic leukemia (t(1;19), with PBX1), childhood leukemia (t(19;19), with TFPT) and acute leukemia (t(12;19), with ZNF384). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, Sep 2011]

Uniprot Description

E2A: a transcription factor that plays major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Heterodimers between E2A and tissue-specific basic helix-loop-helix (bHLH) Dimers bind DNA on E-box motifs: 5'- CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Deletions in E2A have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Two alternatively spliced human isoforms have been described.

Protein type: Oncoprotein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; transcription factor complex; protein complex; cytoplasm; nuclear chromatin; nucleus

Molecular Function: protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; transcription coactivator activity; bHLH transcription factor binding; chromatin binding; mitogen-activated protein kinase kinase kinase binding; transcription factor binding; transcription factor activity

Biological Process: Peyer's patch development; response to drug; protein stabilization; transcription, DNA-dependent; immunoglobulin V(D)J recombination; positive regulation of transcription, DNA-dependent; B cell lineage commitment; T cell differentiation in the thymus; positive regulation of cell cycle; response to lipopolysaccharide; negative regulation of transcription from RNA polymerase II promoter; muscle cell differentiation; regulation of transcription, DNA-dependent; natural killer cell differentiation; B cell differentiation; positive regulation of B cell proliferation; positive regulation of muscle cell differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; cell development