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Western Blot (WB) (Western blot analysis of GDF10 Antibody (Center) in NIH-3T3 cell line lysates (35ug/lane). GDF10 (arrow) was detected using the purified Pab.)

Rabbit GDF10 Polyclonal Antibody | anti-GDF10 antibody

GDF10 Antibody (Center)

Gene Names
GDF10; BMP3B; BMP-3b
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
GDF10; Polyclonal Antibody; GDF10 Antibody (Center); Bone morphogenetic protein 3B; BMP-3B; Bone-inducing protein; BIP; Growth/differentiation factor 10; GDF-10; BMP3B; anti-GDF10 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This GDF10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-344 amino acids from the Central region of human GDF10.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.31 (varies by lot)
Sequence Positions
317-344
Sequence Length
478
Applicable Applications for anti-GDF10 antibody
Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of GDF10 Antibody (Center) in NIH-3T3 cell line lysates (35ug/lane). GDF10 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of GDF10 Antibody (Center) in NIH-3T3 cell line lysates (35ug/lane). GDF10 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of K562 cells using GDF10 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of K562 cells using GDF10 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-GDF10 antibody
GDF10 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice suggest that the protein encoded by this gene plays a role in skeletal morphogenesis.
Product Categories/Family for anti-GDF10 antibody
References
Ducy, P., et al., Kidney Int. 57(6):2207-2214 (2000).
Hino, J., et al., Biochem. Biophys. Res. Commun. 223(2):304-310 (1996).
Cunningham, N.S., et al., Growth Factors 12(2):99-109 (1995).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
53122
NCBI Official Full Name
growth/differentiation factor 10
NCBI Official Synonym Full Names
growth differentiation factor 10
NCBI Official Symbol
GDF10
NCBI Official Synonym Symbols
BMP3B; BMP-3b
NCBI Protein Information
growth/differentiation factor 10
UniProt Protein Name
Growth/differentiation factor 10
UniProt Gene Name
GDF10
UniProt Synonym Gene Names
GDF-10; BMP-3B; BIP
UniProt Entry Name
GDF10_HUMAN

NCBI Description

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice suggest that the protein encoded by this gene plays a role in skeletal morphogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

GDF10: is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice suggest that plays a role in skeletal morphogenesis. [provided by RefSeq, Jul 2008]

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q11.22

Cellular Component: extracellular space

Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: regulation of apoptosis; fat cell differentiation; regulation of MAPKKK cascade; transforming growth factor beta receptor signaling pathway; skeletal development; cell development; growth

Research Articles on GDF10

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Product Notes

The GDF10 gdf10 (Catalog #AAA9214819) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 317-344. The GDF10 Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's GDF10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the GDF10 gdf10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GDF10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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