Mouse glucagon ELISA Kit | GCG elisa kit

Mouse Glucagon, GC ELISA Kit

Cat. Num. AAA705540

• Gene Names: Gcg; Glu; PPG; GLP-1
• Reactivity: Mouse
• Synonyms: glucagon; Mouse Glucagon; GC ELISA Kit; Mouse Glucagon (GC) ELISA Kit; GLP1; GLP2; GRPP; glicentin-related polypeptide; glucagon-like peptide 1; glucagon-like peptide 2; GCG elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse GCG. No significant cross-reactivity or interference between Mouse GCG and analogues was observed.
• Sequence Length: 180

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 1.56 pg/ml-100 pg/ml
• Sensitivity: 0.39 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GCG elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GC bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 33468853
• NCBI GeneID: 14526
• NCBI Accession #: NP_032126.1
• NCBI GenBank Nucleotide #: NM_008100.3
• UniProt Accession #: P55095; Q3UFE9
• Molecular Weight: 20,906 Da
• NCBI Official Full Name: glucagon
• NCBI Official Synonym Full Names: glucagon
• NCBI Official Symbol: Gcg
• NCBI Official Synonym Symbols: Glu; PPG; GLP-1
• NCBI Protein Information: glucagon; glucagon-like peptide I
• UniProt Protein Name: Glucagon
• Protein Family: Glucagon
• UniProt Gene Name: Gcg
• UniProt Synonym Gene Names: GRPP; OXM; OXY; GLP-1; GLP-1(7-37); GLP-1(7-36); GLP-2
• UniProt Entry Name: GLUC_MOUSE

NCBI Description

This gene encodes glucagon, a pancreatic hormone that counteracts the action of insulin in the bloodstream. The encoded protein is processed to generate glucagon and two other glucagon-like peptides, GLP1 and GLP2. Glucagon stimulates gluconeogenesis, glycogenolysis and lipolysis. GLP1 induces secretion of insulin, suppresses glucagon secretion and inhibits feeding. GLP2 induces intestinal absorption of glucose by stimulating the growth of intestinal cells and preventing apoptosis. [provided by RefSeq, Apr 2015]

Uniprot Description

Function: Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia 1 PublicationManual assertion based on experiment in:Ref.5