Pig glucagon ELISA Kit | GCG elisa kit

Pig Glucagon, GC ELISA Kit

Cat. Num. AAA705469

• Reactivity: Pig
• Synonyms: glucagon; Pig Glucagon; GC ELISA Kit; Pig Glucagon (GC) ELISA Kit; GLP1; GLP2; GRPP; glicentin-related polypeptide; glucagon-like peptide 1; glucagon-like peptide 2; GCG elisa kit

• Reactivity: Pig
• Specificity: This assay has high sensitivity and excellent specificity for detection of Pig GCG. No significant cross-reactivity or interference between Pig GCG and analogues was observed.
• Sequence Length: 180

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Competitive
• Detection Range: 125 pg/ml-2000 pg/ml
• Sensitivity: 62.5 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GCG elisa kit

Principle of the Assay: This assay employs the direct competitive inhibition enzyme immunoassay technique. An antibody specific for GC has been pre-coated onto a microplate. Add GC (Standards or samples) to the well, and then add Biotin-conjugated GC. A competitive inhibition reaction is launched between GC (Standards or samples) and Biotin conjugated GC with the GC antibody. Then add the HRP-avidin to each well. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of GC bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 47523706
• NCBI GeneID: 397595
• NCBI Accession #: NP_999489.1
• NCBI GenBank Nucleotide #: NM_214324.1
• UniProt Accession #: P01274; Q864V8
• Molecular Weight: 21,029 Da
• NCBI Official Full Name: glucagon
• NCBI Official Symbol: GCG
• NCBI Protein Information: glucagon; proglucagon
• UniProt Protein Name: Glucagon
• Protein Family: Glucagon
• UniProt Gene Name: GCG
• UniProt Synonym Gene Names: GRPP; OXM; OXY; GLP-1; GLP-1(7-37); GLP-1(7-36); GLP-2
• UniProt Entry Name: GLUC_PIG

Uniprot Description

Function: Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia