GBP1 recombinant protein

GBP1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121189

• Purity: >95% as determined by SDS-PAGE
• Synonyms: GBP1; GBP1 Protein; Human; Recombinant (His Tag); Human GBP1 Protein (His Tag); guanylate binding protein 1; GBP1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Cys589

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met 1
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human GBP1 (AAA35871.1)(Met 1-Cys 589) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for GBP1 recombinant protein

Background: Guanylate-binding protein 1 (GBP-1) is a member of the GBP family whose members are GTPases induced in response to interferon-lambda (IFN-lambda), with seven highly homologous members in humans, termed HuGBP-1 to HuGBP-7. GBP-1 expression is induced by type1 and type2 interferons, including IFN-lambda and also by interleukin-1beta (IL-1beta), IL-1alpha, and tumor necrosis factor-alpha (TNF-alpha). GBP-1 is key to the protective immunity against microbial and viral pathogens. GBP-1 was only secreted from endothelial cells. Secretion occurred without the presence of a leader peptide. Secretion procession is a nonclassical, likely ABC transporter-dependent, pathway and independent of GBP-1 GTPase activity and isoprenylation, and did not require additional interferon-lambda-induced factors. Clinically most important was the detection of significantly increased GBP-1 concentrations in the cerebrospinal fluid of patients with bacterial meningitis as compared to control patients.

Product Categories/Family for GBP1 recombinant protein

NF-kB (NFkB) Pathway

References

Tripal P, et al. (2007) Unique features of different members of the human guanylate-binding protein family. J Interferon Cytokine Res. 27(1): 44-52.Naschberger E, et al. (2006) Human Guanylate Binding Protein-1 Is a Secreted GTPase Present in Increased Concentrations in the Cerebrospinal Fluid of Patients with Bacterial Meningitis. Am J Pathol. 169(3): 1088-99.

NCBI and Uniprot Product Information

• NCBI GI #: 183002
• NCBI GeneID: 2633
• Molecular Weight: 67,931 Da
• NCBI Official Full Name: guanylate binding protein isoform I
• NCBI Official Synonym Full Names: guanylate binding protein 1
• NCBI Official Symbol: GBP1
• NCBI Protein Information: guanylate-binding protein 1
• Protein Family: Guanylate-binding protein

NCBI Description

Guanylate binding protein expression is induced by interferon. Guanylate binding proteins are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP) and are distinguished from the GTP-binding proteins by the presence of 2 binding motifs rather than 3. [provided by RefSeq, Jul 2008]