CD14 recombinant protein

CD14 Protein, Rat, Recombinant (hFc Tag)

Cat. Num. AAA8123426

• Purity: >93% as determined by SDS-PAGE
• Synonyms: CD14; CD14 Protein; Rat; Recombinant (hFc Tag); Rat CD14 Protein (Fc Tag); CD14 molecule; CD14 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >93% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Tyr341

• Species: Rat
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ser 18
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the rat CD14 (Q63691)(Met1-Tyr341) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CD14 recombinant protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 14 (CD14) is a member of the CD system. It takes its name from its inclusion in the CD molecule surface marker proteins. CD14 exists in two forms: a form anchored into the membrane or a soluble form. CD14 was found expressed in macrophages, neutrophil granulocyte and dendritic cells. The major function is to serve as a co-receptor (along with TLR4 and MD-2) for the bacterial lipopolysaccharide (LPS) and other pathogen-associated molecular patterns.

Product Categories/Family for CD14 recombinant protein

NF-kB (NFkB) Pathway

References

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.SD Wright, et al. (1990) CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science. 249 (4975): 1431-3.

NCBI and Uniprot Product Information

• NCBI GI #: 11120688
• NCBI GeneID: 60350
• NCBI Accession #: NP_068512.1
• NCBI GenBank Nucleotide #: NM_021744.1
• UniProt Accession #: Q63691; Q6GT04
• Molecular Weight: 40,054 Da
• NCBI Official Full Name: monocyte differentiation antigen CD14
• NCBI Official Synonym Full Names: CD14 molecule
• NCBI Official Symbol: Cd14
• NCBI Protein Information: monocyte differentiation antigen CD14
• UniProt Protein Name: Monocyte differentiation antigen CD14
• Protein Family: Monocyte differentiation antigen
• UniProt Gene Name: Cd14

NCBI Description

component of the lipopolysaccharide receptor complex; mediates LPS-induced neuroinflammation and inflammatory response [RGD, Feb 2006]

Uniprot Description

Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- ().