Frizzled-10 / FZD10 Recombinant Protein | FZD10 recombinant protein

Recombinant Mouse Frizzled-10 / FZD10 Protein (His tag)

Cat. Num. AAA2546911

• Gene Names: Fzd10; Fz-10
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: Frizzled-10 / FZD10; Recombinant Mouse Frizzled-10 / FZD10 Protein (His tag); Fz-10; FZD10 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 582

• Application Notes: The secreted recombinant mouse FZD10 comprises 152 amino acids and has a predicted molecular mass of 17.4 kDa. As a result of glycosylation, the apparent molecular mass of rm FZD10 is approximately 23 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Ile 22
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for FZD10 recombinant protein

Background: Mouse Frizzled-10, also known as Fz-10, CD350 and FZD10, is a multi-pass membrane protein which belongs to the G-protein coupled receptor Fz/Smo family. Frizzled-10 / FZD10 is abundantly expressed in the cerebellum, followed by cerebral cortex, medulla and spinal cord; very low levels in total brain, frontal lobe, temporal lobe and putamen. It is weakly expressed in adult brain, heart, lung, skeletal muscle, pancreas, spleen and prostate. Frizzled-10 / FZD10 is a receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. Frizzled-10 / FZD10 may also be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

Description: A DNA sequence encoding the N-terminal segment of mouse FZD10 (NP_780493.1) (Met 1-Gly 162) was expressed, fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 28202015
• NCBI GeneID: 93897
• NCBI Accession #: NP_780493.1
• NCBI GenBank Nucleotide #: NM_175284.3
• UniProt Accession #: Q8BKG4
• Molecular Weight: 65,318 Da
• NCBI Official Full Name: frizzled-10
• NCBI Official Synonym Full Names: frizzled class receptor 10
• NCBI Official Symbol: Fzd10
• NCBI Official Synonym Symbols: Fz-10
• NCBI Protein Information: frizzled-10
• UniProt Protein Name: Frizzled-10
• Protein Family: Frizzled
• UniProt Gene Name: Fzd10
• UniProt Synonym Gene Names: Fz10; Fz-10

Uniprot Description

FZD10: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: GPCR, Fz/Smo family; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 5|5 G1.3

Cellular Component: cell surface; cytoplasm; integral to membrane

Molecular Function: G-protein coupled receptor activity; Wnt receptor activity; Wnt-protein binding

Biological Process: positive regulation of GTPase activity; positive regulation of JNK activity; regulation of actin cytoskeleton organization and biogenesis; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin