Immunohistochemistry (IHC)
(Biotinylated (0.1ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
ACAT1 Antibody | anti-ACAT1 antibody
Goat anti-ACAT1 (aa257-269), Biotinylated Antibody
Gene Names
ACAT1; T2; MAT; ACAT; THIL
Reactivity
Human, Mouse, RatExpected from sequence similarity: Cow
Applications
ELISA, Western Blot, Immunofluorescence
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
ACAT1; Goat anti-ACAT1 (aa257-269); Biotinylated Antibody; Goat Anti-ACAT1 (aa257-269) Antibody; acetyl-CoA acetyltransferase 1; ACAT; MAT; T2; THIL; acetoacetyl Coenzyme A thiolase; acetoacetyl-CoA thiolase; acetyl-Coenzyme A acetyltransferase 1; mitochondrial acetoacetyl-CoA thiolase; anti-ACAT1 antibody
Reactivity
Human, Mouse, Rat
Expected from sequence similarity: Cow
Expected from sequence similarity: Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence Length
427
Applicable Applications for anti-ACAT1 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
Pep-ELISA: antibody detection limit dilution 1:8000.
WB: Approx 45kDa band observed in Mouse Liver lysates. Recommended concentration: 0.1-0.3ug/ml.
WB: Approx 45kDa band observed in Mouse Liver lysates. Recommended concentration: 0.1-0.3ug/ml.
Immunogen Sequence
Peptide with sequence C-KRVDFSKVPKLKT., from the internal region of the protein sequence according to NP_000010.1.
Peptide Sequence + C
C-KRVDFSKVPKLKT.
Epitope
Internal Region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Immunohistochemistry (IHC)
(Biotinylated (0.1ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Immunohistochemistry (IHC)
(Biotinylated (0.1ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44.8kDa
NCBI Official Full Name
acetyl-CoA acetyltransferase, mitochondrial
NCBI Official Synonym Full Names
acetyl-CoA acetyltransferase 1
NCBI Official Symbol
ACAT1
NCBI Official Synonym Symbols
T2; MAT; ACAT; THIL
NCBI Protein Information
acetyl-CoA acetyltransferase, mitochondrial
UniProt Protein Name
Acetyl-CoA acetyltransferase, mitochondrial
UniProt Gene Name
ACAT1
UniProt Synonym Gene Names
ACAT; MAT
NCBI Description
This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]
Uniprot Description
Plays a major role in ketone body metabolism.
Research Articles on ACAT1
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Product Notes
The ACAT1 acat1 (Catalog #AAA423739) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ACAT1 (aa257-269), Biotinylated Antibody reacts with Human, Mouse, Rat Expected from sequence similarity: Cow and may cross-react with other species as described in the data sheet. AAA Biotech's ACAT1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). Pep-ELISA: antibody detection limit dilution 1:8000. WB: Approx 45kDa band observed in Mouse Liver lysates. Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the ACAT1 acat1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACAT1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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