Rabbit anti-Human UQCRB Polyclonal Antibody | anti-UQCRB antibody

UQCRB, ID (UQCRB, UQBP, Cytochrome b-c1 complex subunit 7, Complex III subunit 7, Complex III subunit VII, QP-C, Ubiquinol-cytochrome c reductase complex 14kD protein)

Cat. Num. AAA6011077

• Gene Names: UQCRB; QPC; QCR7; QP-C; UQBC; UQBP; UQPC; UQCR6; MC3DN3
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: UQCRB; Polyclonal Antibody; ID (UQCRB; UQBP; Cytochrome b-c1 complex subunit 7; Complex III subunit 7; Complex III subunit VII; QP-C; Ubiquinol-cytochrome c reductase complex 14kD protein); Anti -UQCRB; anti-UQCRB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-UQCRB antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50
• Immunogen: UQCRB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-47 amino acids from the Central region of human UQCRB.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(UQCRB Antibody (Center) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the UQCRB antibody detected the UQCRB protein (arrow).)

Immunohistochemistry (IHC)

(UQCRB Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UQCRB Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-UQCRB antibody

This gene encodes a protein which is part of the ubiquinol-cytochrome c oxidoreductase complex which contains ten nuclear-encoded and one mitochondrial-encoded subunits. The encoded protein binds ubiquinone and participates in the transfer of electrons when ubiquinone is bound. Mutations in this gene are associated with mitochondrial complex III deficiency. A pseudogene has been described on the X chromosome.

Product Categories/Family for anti-UQCRB antibody

Antibodies; Abs to Enzymes; Cytochrome

NCBI and Uniprot Product Information

• NCBI GI #: 49457388
• NCBI GeneID: 7381
• UniProt Accession #: P14927; Q6FGD1; E5RJU0
• Molecular Weight: 13,530 Da
• NCBI Official Full Name: UQCRB
• NCBI Official Synonym Full Names: ubiquinol-cytochrome c reductase binding protein
• NCBI Official Symbol: UQCRB
• NCBI Official Synonym Symbols: QPC; QCR7; QP-C; UQBC; UQBP; UQPC; UQCR6; MC3DN3
• NCBI Protein Information: cytochrome b-c1 complex subunit 7; complex III subunit 7; complex III subunit VII; mitochondrial ubiquinone-binding protein; ubiquinol-cytochrome c reductase complex 14 kDa protein; ubiquinol-cytochrome c reductase, complex III subunit VI
• UniProt Protein Name: Cytochrome b-c1 complex subunit 7
• Protein Family: Cytochrome b-c1 complex
• UniProt Gene Name: UQCRB
• UniProt Synonym Gene Names: UQBP
• UniProt Entry Name: QCR7_HUMAN

NCBI Description

This gene encodes a subunit of the ubiquinol-cytochrome c oxidoreductase complex, which consists of one mitochondrial-encoded and 10 nuclear-encoded subunits. The protein encoded by this gene binds ubiquinone and participates in the transfer of electrons when ubiquinone is bound. This protein plays an important role in hypoxia-induced angiogenesis through mitochondrial reactive oxygen species-mediated signaling. Mutations in this gene are associated with mitochondrial complex III deficiency. Alternatively spliced transcript variants have been found for this gene. Related pseudogenes have been identified on chromosomes 1, 5 and X. [provided by RefSeq, Dec 2011]

Uniprot Description

UQCRB: This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This component is involved in redox-linked proton pumping. Defects in UQCRB are a cause of mitochondrial complex III deficiency (MT-C3D). A disorder of the mitochondrial respiratory chain resulting in a highly variable phenotype depending on which tissues are affected. Clinical features include mitochondrial encephalopathy, psychomotor retardation, ataxia, severe failure to thrive, liver dysfunction, renal tubulopathy, muscle weakness and exercise intolerance. Belongs to the UQCRB/QCR7 family.

Protein type: Energy Metabolism - oxidative phosphorylation; EC 1.10.2.2; Oxidoreductase; Mitochondrial

Chromosomal Location of Human Ortholog: 8q22

Cellular Component: mitochondrial inner membrane; mitochondrial respiratory chain complex III; mitochondrial respiratory chain

Biological Process: cellular metabolic process; aerobic respiration; oxidative phosphorylation; mitochondrial electron transport, ubiquinol to cytochrome c

Disease: Mitochondrial Complex Iii Deficiency, Nuclear Type 3