Phosphoenolpyruvate synthase (ppsA) Recombinant Protein | ppsA recombinant protein

Recombinant Escherichia coli Phosphoenolpyruvate synthase (ppsA)

Cat. Num. AAA1132998

• Gene Names: ppsA; ECK1700; JW1692; pps
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Phosphoenolpyruvate synthase (ppsA); Recombinant Escherichia coli Phosphoenolpyruvate synthase (ppsA); Pyruvate; water dikinase; ppsA recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 2-792aa; Full Length of Mature Protein
• Sequence: SNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVSVPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQPELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAVKHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHGKQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVLVTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENVTVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATLGAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFALECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIPSNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRAAKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK
• Sequence Length: 792

• Production Note: Special Offer: The E Coli host-expressed protein is manufactured from a stock plasmid containing the protein gene. E Colihost-expressed protein is stocked in different unit sizes ranging from as small as 10 ug to as large as 1 mg. Bulk inventory is also available. The E Coli host-expressed protein has been ordered over and over again by researchers and has stood the test of time as both a robust protein and important target for the research community. It is part of our new program to make our most popular protein targets and corresponding hosts available in expanded unit sizes and with a quick processing time. Select E Coli host-expressed protein for the fastest delivery among all hosts. Please contact our technical support team or email to [email protected] for more details.
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for ppsA recombinant protein

Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate.

References

The cloning and sequence of the E. coli pps gene.Holzschu D.L., McElver J.A., Liao C.C., Berry A. Cloning and nucleotide sequence of the Escherichia coli K-12 ppsA gene, encoding PEP synthase.Niersbach M., Kreuzaler F., Geerse R.H., Postma P.W., Hirsch H.J.Mol. Gen. Genet. 231:332-336(1992) A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.DNA Res. 3:363-377(1996) The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) The mechanism of the phosphoenolpyruvate synthase reaction.Cooper R.A., Kornberg H.L.Biochim. Biophys. Acta 141:211-213(1967) Phosphoenolpyruvate synthetase of Escherichia coli. Purification, some properties, and the role of divalent metal ions.Berman K.M., Cohn M.J. Biol. Chem. 245:5309-5318(1970) Phosphoenolypyruvate synthetase of Escherichia coli molecular weight, subunit composition, and identification of phosphohistidine in phosphoenzyme intermediate.Narindrasorasak S., Bridger W.A.J. Biol. Chem. 252:3121-3127(1977) Escherichia coli proteome analysis using the gene-protein database.VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.Electrophoresis 18:1243-1251(1997) Cloning and characterization of Escherichia coli DUF299 a bifunctional ADP-dependent kinase--Pi-dependent pyrophosphorylase from bacteria.Burnell J.N.BMC Biochem. 11:1-1(2010)

NCBI and Uniprot Product Information

• NCBI GI #: 16129658
• NCBI GeneID: 946209
• NCBI Accession #: NP_416217.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P23538
• Molecular Weight: 103.3 kDa
• NCBI Official Full Name: phosphoenolpyruvate synthase
• NCBI Official Symbol: ppsA
• NCBI Official Synonym Symbols: ECK1700; JW1692; pps
• NCBI Protein Information: phosphoenolpyruvate synthase
• UniProt Protein Name: Phosphoenolpyruvate synthase
• Protein Family: Plipastatin synthase
• UniProt Gene Name: ppsA
• UniProt Synonym Gene Names: pps; PEP synthase
• UniProt Entry Name: PPSA_ECOLI

Uniprot Description

Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate.