Mouse Flt-3ligand ELISA Kit | FLT3LG elisa kit

Mouse Flt-3ligand PicoKine ELISA Kit

Cat. Num. AAA177397

• Gene Names: Flt3l; Ly72L; Flt3lg
• Reactivity: Mouse
• Synonyms: Flt-3ligand; Mouse Flt-3ligand PicoKine ELISA Kit; Fms-related tyrosine kinase 3 ligand; fms-related tyrosine kinase 3 ligand; Flt 3 ligand; Flt 3L; Flt3 L; FLT3 LG; Flt3 ligand; Flt3L; FLT3L_HUMAN; Flt3lg; Fms related tyrosine kinase 3 ligand; SL cytokine; FLT3LG elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse Flt-3ligand
• Sequence Length: 232

• Samples: Cell culture supernates and serum
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: < 10pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse Flt-3ligand PicoKine ELISA Kit standard curve )

Related Product Information for FLT3LG elisa kit

Principle of the assay: mouse Flt-3ligand ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Flt-3ligand has been precoated onto 96-well plates. Standards(NSO, G27-R188) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Flt-3ligand is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Flt-3ligand amount of sample captured in plate.
Background: Fms-related tyrosine kinase 3 ligand (FLT3LG), also known as Flt-3ligand or FL, is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 19q13.3. Flt-3ligand is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1). In synergy with other growth factors, Flt-3ligand stimulates the proliferation and differentiation of various blood cell progenitors. Besides that, Flt-3ligand can control the development of Dendritic cells (DCs) and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Flt-3ligand also can enhance the response of stem and primitive progenitor cells to other growth factors to generate all myeloid lineages except erythroid cells.

NCBI and Uniprot Product Information

• NCBI GI #: 1706819
• NCBI GeneID: 14256
• UniProt Accession #: P49772; Q64085
• Molecular Weight: 28,259 Da
• NCBI Official Full Name: Fms-related tyrosine kinase 3 ligand
• NCBI Official Synonym Full Names: FMS-like tyrosine kinase 3 ligand
• NCBI Official Symbol: Flt3l
• NCBI Official Synonym Symbols: Ly72L; Flt3lg
• NCBI Protein Information: fms-related tyrosine kinase 3 ligand; flt3 ligand
• UniProt Protein Name: Fms-related tyrosine kinase 3 ligand
• Protein Family: Fms-related tyrosine kinase
• UniProt Gene Name: Flt3lg
• UniProt Synonym Gene Names: Flt3l; Flt3 ligand; Flt3L
• UniProt Entry Name: FLT3L_MOUSE

Uniprot Description

FLT3LG: Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligand, receptor tyrosine kinase; Cytokine; Membrane protein, integral

Cellular Component: extracellular space; cell surface; membrane; integral to membrane; extracellular region; plasma membrane

Molecular Function: protein homodimerization activity; cytokine activity; receptor tyrosine kinase binding

Biological Process: regulation of myeloid dendritic cell activation; positive regulation of natural killer cell differentiation; lymphocyte differentiation; embryonic hemopoiesis; positive regulation of cell proliferation; positive regulation of protein amino acid phosphorylation; positive regulation of myoblast differentiation