SDS-Page
SRP14 recombinant protein
SRP14, 1-136aa, Human, His tag, E Coli
Gene Names
SRP14; ALURBP
Applications
SDS-Page
Purity
> 85% by SDS-PAGE
Synonyms
SRP14; 1-136aa; Human; His tag; E Coli; Signal recognition particle 14 kDa protein; DKFZp468D2027; SRP14 recombinant protein
Host
E Coli
Purity/Purification
> 85% by SDS-PAGE
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Concentration
1.0 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMVLLES EQFLTELTRL FQKCRTSGSV YITLKKYDGR TKPIPKKGTV EGFEPADNKC LLRATDGKKK ISTVVSSKEV NKFQMAYSNL LRANMDGLKK RDKKNKTKKT KAAAAAAAAA PAAAATAATT AATTAATAAQ
Sequence Length
136
Applicable Applications for SRP14 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for SRP14 recombinant protein
SRP14 is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum. The Alu domain of SRP comprises the heterodimer of the SRP9 and SRP14 proteins, which are bound to the 5' and 3' terminal sequences of SRP RNA. SRP9/14 binding may be crucial to the transcription, maturation, nucleolus localization and transport of SRP RNA. Recombinant human SRP14 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.
Product Categories/Family for SRP14 recombinant protein
References
Chang D.Y., et al. (1994), Mol. Cell. Biol, 14:3949-3959.; Hsu K., et al. (1995). J. Biol. Chem., 270:10179-10186
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17.1 kDa (160aa) confirmed by MALDI-TOF (molecular weight on SDS-PAGE will appear higher)
NCBI Official Full Name
signal recognition particle 14kDa (homologous Alu RNA binding protein)
NCBI Official Synonym Full Names
signal recognition particle 14
NCBI Official Symbol
SRP14
NCBI Official Synonym Symbols
ALURBP
NCBI Protein Information
signal recognition particle 14 kDa protein
UniProt Protein Name
Signal recognition particle 14 kDa protein
Protein Family
UniProt Gene Name
SRP14
UniProt Synonym Gene Names
SRP14
Uniprot Description
Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. The complex of SRP9 and SRP14 is required for SRP RNA binding.
Research Articles on SRP14
Similar Products
Product Notes
The SRP14 srp14 (Catalog #AAA203790) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's SRP14 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the SRP14 srp14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSHMVLLES EQFLTELTRL FQKCRTSGSV YITLKKYDGR TKPIPKKGTV EGFEPADNKC LLRATDGKKK ISTVVSSKEV NKFQMAYSNL LRANMDGLKK RDKKNKTKKT KAAAAAAAAA PAAAATAATT AATTAATAAQ. It is sometimes possible for the material contained within the vial of "SRP14, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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