bFGF recombinant protein

Rat bFGF protein

Cat. Num. AAA9719062

• Gene Names: Fgf2; bFGF; Fgf-2
• Purity: >95% as determined by SDS-PAGE
• Synonyms: bFGF; Rat bFGF protein; Fibroblast Growth Factor 2; FGF-2; Basic Fibroblast Growth Factor; Heparin-Binding Growth Factor 2; HBGF-2; FGF2; FGFB; bFGF recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20 mM Tris, 150 mM NaCl, pH8.0.
• Sequence Positions: 145aa
• Sequence: Ala11-Ser154

• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method.
• Protein Construction: Amino acid sequence derived from Rat FGF2 isoform (P13109) (Ala11-Ser154) was expressed.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Rat bFGF protein using the buffer we provided not less than 100 ug/mL, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Lyophilized Rat bFGF protein product should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Related Product Information for bFGF recombinant protein

Recombinant Rat bFGF protein expressed in E Coli.
Background: Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the fibroblast growth factor (FGF) family. FGF basic modulates such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, neuronal function and neural degeneration. Transgenic over-expression of FGF basic results in excessive proliferation and angiogenesis reminiscent of a variety of pathological conditions.

Product Categories/Family for bFGF recombinant protein

Cell Culture

NCBI and Uniprot Product Information

• NCBI GI #: 9506599
• NCBI GeneID: 54250
• NCBI Accession #: NP_062178.1
• NCBI GenBank Nucleotide #: NM_019305.2
• UniProt Accession #: P13109
• Molecular Weight: 17,139 Da
• NCBI Official Full Name: fibroblast growth factor 2
• NCBI Official Synonym Full Names: fibroblast growth factor 2
• NCBI Official Symbol: Fgf2
• NCBI Official Synonym Symbols: bFGF; Fgf-2
• NCBI Protein Information: fibroblast growth factor 2; HBGF-2; basic fibroblast growth factor; heparin-binding growth factor 2
• UniProt Protein Name: Fibroblast growth factor 2
• UniProt Gene Name: Fgf2
• UniProt Synonym Gene Names: Fgf-2; FGF-2; bFGF; HBGF-2
• UniProt Entry Name: FGF2_RAT

NCBI Description

activates the MAP kinase signaling pathway; plays a role in synaptic transmission; induces cell proliferation [RGD, Feb 2006]

Uniprot Description

FGF2: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2. Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue. Belongs to the heparin-binding growth factors family. 4 isoforms of the human protein are produced by alternative initiation.

Protein type: Motility/polarity/chemotaxis; Activator

Cellular Component: extracellular space; cytoplasm; cytosol; nucleus

Molecular Function: heparin binding; protein binding; ligand-dependent nuclear receptor transcription coactivator activity; growth factor activity; cytokine activity; chemoattractant activity; fibroblast growth factor receptor binding

Biological Process: wound healing; activation of MAPKK activity; regulation of cell cycle; positive regulation of transcription, DNA-dependent; positive regulation of smooth muscle cell proliferation; adrenocorticotropin hormone secreting cell differentiation; thyroid stimulating hormone secreting cell differentiation; hyaluronan catabolic process; growth factor dependent regulation of satellite cell proliferation; negative regulation of cell proliferation; embryonic development ending in birth or egg hatching; positive regulation of MAP kinase activity; glial cell differentiation; substantia nigra development; positive chemotaxis; induction of an organ; positive regulation of cell proliferation; regulation of retinal cell programmed cell death; angiogenesis; response to axon injury; aging; positive regulation of cardiac muscle cell proliferation; positive regulation of granule cell precursor proliferation; fibroblast growth factor receptor signaling pathway; positive regulation of cell fate specification; positive regulation of blood vessel endothelial cell migration; positive regulation of phosphoinositide 3-kinase activity; negative regulation of blood vessel endothelial cell migration; positive regulation of protein kinase B signaling cascade; positive regulation of osteoblast differentiation; stem cell development; positive regulation of angiogenesis; cell migration during sprouting angiogenesis; ureteric bud branching; release of sequestered calcium ion into cytosol; positive regulation of cell division; phosphatidylinositol biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; positive regulation of cell differentiation; inositol phosphate biosynthetic process; positive regulation of epithelial cell proliferation; lung development