FGF-2 (basic) Active Protein | FGF-2 active protein

Rat FGF-2 (basic)

Cat. Num. AAA691862

• Gene Names: Fgf2; bFGF; Fgf-2
• Reactivity: Rat
• Purity: > 98% by SDS-PAGE & silver stain
• Synonyms: FGF-2 (basic); Rat FGF-2 (basic); Recombinant Rat Fibroblast Growth Factor-2; basic Fibroblast growth factor (bFGF); Heparin binding growth factor-2 (HBGF-2); FGF-2 active protein

• Host: E Coli
• Reactivity: Rat
• Purity/Purification: > 98% by SDS-PAGE & silver stain
• Form/Format: Freeze dried
• Sequence: N-Terminal Sequence: ALPEDGGGAFPP
• Sequence Length: 145

• Endotoxin Level: < 0.1ng per ug of rat FGF-2
• Buffer: 0,5X PBS
• Preparation and Storage: The lyophilized rat FGF-2, though stable at room temperature, is best stored in working aliquots at -20 degree C to -70 degree C

Testing Data

Testing Data #2

Related Product Information for FGF-2 active protein

The FGF family is composed of at least 23 polypeptides that show a variety of biological activities towards cells of mesenchymal, neuronal and epithelial origin. All members are heparin-binding growth factors (HB-GF). Until the structure of basic fibroblast growth factor (bFGF/FGF-2) was determined, a number of synonyms were used to describe this growth factor. As is often the case, the nomenclature reflected the observed activities reported by individual groups. Basic FGF has been reported as leukemia growth factor, macrophage growth factor, endothelial growth factor and tumor angiogenesis factor. The eventual isolation and characterization of bFGF was done from soluble brain extracts. bFGF was found to have a molecular mass of 16.5 kDa and to be 154 amino acids in length. Interestingly, bFGF contains no hydrophobic leader sequence previously thought to be required for cell secretion. Basic FGF bears 55% homology to acidic FGF and also seems to exist in three forms: the 154 amino-acid form and two other truncated versions of 146 and 131 amino acids lacking the N-terminal 9 and 24 residues. Acidic and basic FGF compete for the binding to 125 kDa and 145 kDa receptor species. However, acidic FGF has a higher affinity for the 125 kDa species, while basic FGF has a higher affinity for the 145 kDa species. FGF receptor activation leads to the activation of MAP kinase and protein kinase C. FGF's induce the proliferative response in cells derived from mesoderm and neuroectoderm. Perhaps one of the most potentially significant applications of bFGF is related to its reported ability to induce angiogenesis. The cDNA of native rat FGF-2 (Ala11-Ser154) was cloned from total RNA derived from a rat embryo using standard protocols.Bitte hier Text zur Produktinformation eingeben. Hinweis: Bei copy/paste Prozessen auf Vollstandigkeit uberprufen.

Product Categories/Family for FGF-2 active protein

Cytokines & Growth Factors

References

1. Hisaoka K et al, J Biol Chem 286, 2011 2. Florkiewicz RZ et al, Biochem Biophys Res Commun 409, 2011 3. Sun Y et al, J Cell Sci 124, 2011 4. Turner CA et al, Proc Natl Acad Sci 108, 2011 5. Eckenstein FP et al, Biochem Pharmacol 47, 1994 6. el-Husseini AE, Biochim Biophys Acta 1131, 1992 7. Fujimoto K et al, Biochem Biophys Res Commun 180, 1991 8. Shimasaki S et al, Biochem Biophys Res Commun 157, 1988 9. Kurokawa et al, Nucleic Acids Res 16, 1988

NCBI and Uniprot Product Information

• NCBI GI #: 9506599
• NCBI GeneID: 54250
• NCBI Accession #: NP_062178.1
• NCBI GenBank Nucleotide #: NM_019305.2
• UniProt Accession #: P13109
• Molecular Weight: 16,34 kDa
• NCBI Official Full Name: fibroblast growth factor 2
• NCBI Official Synonym Full Names: fibroblast growth factor 2
• NCBI Official Symbol: Fgf2
• NCBI Official Synonym Symbols: bFGF; Fgf-2
• NCBI Protein Information: fibroblast growth factor 2; HBGF-2; basic fibroblast growth factor; heparin-binding growth factor 2
• UniProt Protein Name: Fibroblast growth factor 2
• UniProt Gene Name: Fgf2
• UniProt Synonym Gene Names: Fgf-2; FGF-2; bFGF; HBGF-2
• UniProt Entry Name: FGF2_RAT

NCBI Description

activates the MAP kinase signaling pathway; plays a role in synaptic transmission; induces cell proliferation [RGD, Feb 2006]

Uniprot Description

Function: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro

By similarity.

Subunit structure: Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2

By similarity.

Subcellular location: Secreted

By similarity. Nucleus

By similarity. Note: Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane

By similarity. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57

By similarity.

Tissue specificity: Found in all tissues examined.

Post-translational modification: Phosphorylation at Tyr-81 regulates FGF2 unconventional secretion

By similarity.

Sequence similarities: Belongs to the heparin-binding growth factors family.