Rabbit anti-Mouse Map2k6 Polyclonal Antibody | anti-Map2k6 antibody

Map2k6, NT (Map2k6, Prkmk6, Sapkk3, Dual specificity mitogen-activated protein kinase kinase 6, MAPK/ERK kinase 6, SAPKK3) (Azide free) (HRP)

Cat. Num. AAA6318521

• Gene Names: Map2k6; MEK6; MKK6; MAPKK6; Prkmk6; SAPKK3; MAPKK 6
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Map2k6; Polyclonal Antibody; NT (Map2k6; Prkmk6; Sapkk3; Dual specificity mitogen-activated protein kinase kinase 6; MAPK/ERK kinase 6; SAPKK3) (Azide free) (HRP); SAPKK3; anti-Map2k6 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-Map2k6 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Map2k6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-57 amino acids from the N-terminal region of Mouse Map2k6.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Map2k6 Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Map2k6 antibody detected the Map2k6 protein (arrow).)

Related Product Information for anti-Map2k6 antibody

Map2k6 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.

Product Categories/Family for anti-Map2k6 antibody

Antibodies; Protein Kinases

References

Remy, G., et al. Cell. Signal. 22(4):660-667(2010). Sorkin, L.S., et al. Neuroscience 162(2):462-471(2009). Yoshizawa, T., et al. J. Immunol. 183(2):1360-1367(2009). Bulat, N., et al. J. Lipid Res. 50(1):81-89(2009). Yamashita, T., et al. Biochem. Biophys. Res. Commun. 365(2):252-257(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 33859654
• NCBI GeneID: 26399
• NCBI Accession #: NP_036073.1
• NCBI GenBank Nucleotide #: NM_011943.3
• UniProt Accession #: P70236
• Molecular Weight: 37,432 Da
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 6 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 6
• NCBI Official Symbol: Map2k6
• NCBI Official Synonym Symbols: MEK6; MKK6; MAPKK6; Prkmk6; SAPKK3; MAPKK 6
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 6
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 6
• Protein Family: Dual specificity mitogen-activated protein kinase kinase
• UniProt Gene Name: Map2k6
• UniProt Synonym Gene Names: Prkmk6; Sapkk3; MAP kinase kinase 6; MAPKK 6; MEK 6

Uniprot Description

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. With MAP3K3/MKK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinases p38 MAPK11, MAPK12, MAPK13 and MAPK14 and plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. Especially, MAP2K3/MKK3 and MAP2K6/MKK6 are both essential for the activation of MAPK11 and MAPK13 induced by environmental stress, whereas MAP2K6/MKK6 is the major MAPK11 activator in response to TNF. MAP2K6/MKK6 also phosphorylates and activates PAK6. The p38 MAP kinase signal transduction pathway leads to direct activation of transcription factors. Nuclear targets of p38 MAP kinase include the transcription factors ATF2 and ELK1. Within the p38 MAPK signal transduction pathway, MAP3K6/MKK6 mediates phosphorylation of STAT4 through MAPK14 activation, and is therefore required for STAT4 activation and STAT4-regulated gene expression in response to IL-12 stimulation. The pathway is also crucial for IL-6-induced SOCS3 expression and down-regulation of IL-6-mediated gene induction; and for IFNG-dependent gene transcription. Has a role in osteoclast differentiation through NF-kappa-B transactivation by TNFSF11, and in endochondral ossification and since SOX9 is another likely downstream target of the p38 MAPK pathway. MAP2K6/MKK6 mediates apoptotic cell death in thymocytes. Acts also as a regulator for melanocytes dendricity, through the modulation of Rho family GTPases.