Rat Adenosine A2a Receptor ELISA Kit | ADORA2a elisa kit

Rat Adenosine A2a Receptor ELISA Kit

Cat. Num. AAA761772

• Gene Names: ADORA2A; A2aR; RDC8; ADORA2
• Reactivity: Rat
• Synonyms: Adenosine A2a Receptor; Rat Adenosine A2a Receptor ELISA Kit; ADORA2a; ADORA2a elisa kit

• Reactivity: Rat
• Sequence Length: 412

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for ADORA2a elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat ADORA2a antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat ADORA2a antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat ADORA2a amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofRat ADORA2a can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 119580062
• NCBI GeneID: 135
• Molecular Weight: 44,707 Da
• NCBI Official Full Name: adenosine A2a receptor
• NCBI Official Synonym Full Names: adenosine A2a receptor
• NCBI Official Symbol: ADORA2A
• NCBI Official Synonym Symbols: A2aR; RDC8; ADORA2
• NCBI Protein Information: adenosine receptor A2a
• UniProt Protein Name: Adenosine receptor A2a
• Protein Family: Adenosine receptor
• UniProt Gene Name: ADORA2A
• UniProt Synonym Gene Names: ADORA2
• UniProt Entry Name: AA2AR_HUMAN

NCBI Description

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily, which is subdivided into classes and subtypes. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein, an adenosine receptor of A2A subtype, uses adenosine as the preferred endogenous agonist and preferentially interacts with the G(s) and G(olf) family of G proteins to increase intracellular cAMP levels. It plays an important role in many biological functions, such as cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. It has been implicated in pathophysiological conditions such as inflammatory diseases and neurodegenerative disorders. Alternative splicing results in multiple transcript variants. A read-through transcript composed of the upstream SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding. [provided by RefSeq, Jun 2013]

Uniprot Description

ADORA2A: one of several receptor subtypes for adenosine. A G-protein coupled receptor. Activation is mediated by G proteins which activate adenylyl cyclase. Abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.

Protein type: Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1; Membrane protein, integral

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: integral to plasma membrane; membrane; plasma membrane

Molecular Function: adenosine receptor activity, G-protein coupled; enzyme binding; identical protein binding; protein binding

Biological Process: adenosine receptor signaling pathway; adenylate cyclase activation; apoptosis; blood circulation; blood coagulation; cAMP biosynthetic process; cell-cell signaling; cellular defense response; cellular protein metabolic process; central nervous system development; G-protein signaling, coupled to cAMP nucleotide second messenger; inflammatory response; nerve growth factor receptor signaling pathway; phagocytosis; sensory perception; transmembrane receptor protein tyrosine kinase signaling pathway