Rat FAS-L ELISA Kit | FASLG elisa kit

Rat FAS-L PicoKine ELISA Kit

Cat. Num. AAA177256

• Reactivity: Rat
• Synonyms: FAS-L; Rat FAS-L PicoKine ELISA Kit; Tumor necrosis factor ligand superfamily member 6; Fas ligand (TNF superfamily; member 6); Apoptosis (APO 1) antigen ligand 1; Apoptosis antigen ligand; APT1LG1; APTL; CD178; CD95 L; CD95 ligand; CD95L; CD95L protein; Fas antigen ligand; FASL; FASLG; TNF superfamily member 6; TNFSF6; Tumor necrosis factor (ligand) superfamily member 6; FASLG elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant Rat Faslg
• Sequence Length: 278

• Samples: Cell culture supernatants, serum and plasma (heparin, edta, citrate)
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FASLG elisa kit

Principle of the Assay: The Picokine™ Rat Faslg Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Faslg with a 96-well strip plate that is pre-coated with antibody specific for Faslg. The detection antibody is a biotinylated antibody specific for Faslg. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Rat Faslg with immunogen: Expression system for standard: NSO; Immunogen sequence: Q127-L278. The kit is analytically validated with ready to use reagents. To measure Rat Faslg, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Rat Faslg in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Faslg in the sample.

Background/Introduction: FAS Ligand(FASL) is a 40 kDa type II membrane protein belonging to the tumor necrosis factor family, which induces apoptosis by binding to its receptor, Fas. The human FasL gene consists of approximately 8.0 kb and is split into four exons. This gene consists of 281 amino acids with a calculated M(r) of 31,759 and was mapped on chromosome 1q23. It has an identity of 76.9% at the amino acid sequence level with mouse FasL. The FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis. FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis. This system may also play a role in modulating the genetic susceptibility of mouse strains to develop T-cell lymphoblastic lymphomas.