Mouse FASL ELISA Kit | FASLG elisa kit

Mouse FASL PicoKine ELISA Kit

Cat. Num. AAA177121

• Gene Names: Fas; lpr; APO1; APT1; CD95; TNFR6; Tnfrsf6; AI196731
• Reactivity: Mouse
• Synonyms: FASL; Mouse FASL PicoKine ELISA Kit; Tumor necrosis factor ligand superfamily member 6; Fas ligand (TNF superfamily; member 6); Apoptosis (APO 1) antigen ligand 1; Apoptosis antigen ligand; APT1LG1; APTL; CD178; CD95 L; CD95 ligand; CD95L; CD95L protein; Fas antigen ligand; FASLG; TNF superfamily member 6; TNFSF6; Tumor necrosis factor (ligand) superfamily member 6; FASLG elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse FAS-L
• Sequence Length: 327

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: < 10pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse FASL PicoKine ELISA Kit standard curve )

Related Product Information for FASLG elisa kit

Principle of the assay: mouse FAS-L ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for FAS-L has been precoated onto 96-well plates. Standards(NSO, P132-L279) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FAS-L is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse FAS-L amount of sample captured in plate.
Background: FAS Ligand (FASL) is a 40 kDa type II membrane protein belonging to the tumor necrosis factor family, which induces apoptosis by binding to its receptor, Fas. The human FasL gene consists of approximately 8.0 kb and is split into four exons. This gene consists of 281 amino acids with a calculated M(r) of 31,759 and was mapped on chromosome 1q23. It has an identity of 76.9% at the amino acid sequence level with mouse FasL. The FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis. FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis. This system may also play a role in modulating the genetic susceptibility of mouse strains to develop T-cell lymphoblastic lymphomas.

NCBI and Uniprot Product Information

• NCBI GI #: 342187056
• NCBI GeneID: 14102
• UniProt Accession #: P25446; Q6GT31; Q9DCQ1
• Molecular Weight: 37,437 Da
• NCBI Official Full Name: Tumor necrosis factor receptor superfamily member 6
• NCBI Official Synonym Full Names: Fas (TNF receptor superfamily member 6)
• NCBI Official Symbol: Fas
• NCBI Official Synonym Symbols: lpr; APO1; APT1; CD95; TNFR6; Tnfrsf6; AI196731
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 6; Fas antigen; apo-1 antigen; FASLG receptor; lymphoproliferation; Fas (TNF receptor superfamily member); apoptosis-mediating surface antigen FAS
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 6
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: Fas
• UniProt Synonym Gene Names: Apt1; Tnfrsf6
• UniProt Entry Name: TNR6_MOUSE

Uniprot Description

FAS: Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). Binds DAXX. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD. Binds RIPK1 and FAIM2. Interacts with BRE and FEM1B. Interacts with FADD. Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine; Cell surface; Apoptosis

Cellular Component: extracellular space; neuron projection; cell surface; extracellular region; integral to membrane; CD95 death-inducing signaling complex; lipid raft; secretory granule; membrane; cell soma; perinuclear region of cytoplasm; apical plasma membrane; cytoplasm; plasma membrane; nucleus; sarcolemma; external side of plasma membrane

Molecular Function: identical protein binding; protein binding; tumor necrosis factor receptor activity; transmembrane receptor activity; protease binding; protein complex binding; kinase binding

Biological Process: renal system process; apoptosis; positive regulation of apoptosis; response to toxin; regulation of lymphocyte differentiation; response to glucocorticoid stimulus; positive regulation of caspase activity; signal transduction; regulation of apoptosis; tumor necrosis factor-mediated signaling pathway; T cell homeostasis; immunoglobulin production; activated T cell apoptosis; protein homooligomerization; spleen development; regulation of myeloid cell differentiation; transformed cell apoptosis; positive regulation of protein homooligomerization; negative regulation of B cell activation; negative thymic T cell selection; regulation of cell proliferation; dendrite regeneration; inflammatory cell apoptosis; B cell mediated immunity; neuron apoptosis; induction of apoptosis via death domain receptors; regulation of gene expression; immune response; gene expression; negative regulation of apoptosis