Human FASL ELISA Kit | FASLG elisa kit

Human FASL PicoKine ELISA Kit

Cat. Num. AAA175984

• Gene Names: Fasl; gld; CD178; CD95L; Fas-L; Faslg; CD95-L; Tnfsf6; APT1LG1
• Reactivity: Human
• Synonyms: FASL; Human FASL PicoKine ELISA Kit; Tumor necrosis factor ligand superfamily member 6; Fas ligand (TNF superfamily; member 6); Apoptosis (APO 1) antigen ligand 1; Apoptosis antigen ligand; APT1LG1; APTL; CD178; CD95 L; CD95 ligand; CD95L; CD95L protein; Fas antigen ligand; FASLG; TNF superfamily member 6; TNFSF6; Tumor necrosis factor (ligand) superfamily member 6; FASLG elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human FASL
• Sequence Length: 131

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
• Assay Type: Sandwich
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: < 2pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human FASL PicoKine ELISA Kit standard curve )

Related Product Information for FASLG elisa kit

Principle of the assay: human FASL ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FASL has been precoated onto 96-well plates. Standards (CHO,P134-L281) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FASL is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FASL amount of sample captured in plate.
Background: Fas ligand (FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. Its binding with its receptor induces apoptosis. The human FASL gene consists of approximately 8 kb and is split into 4 exons. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand or FasL is a homotrimeric type II transmembrane protein. It signals through trimerization of FasR, which spans the membrane of the "target" cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7.

NCBI and Uniprot Product Information

• NCBI GI #: 81903309
• NCBI GeneID: 14103
• UniProt Accession #: Q99PH8; P41047
• Molecular Weight: 14,538 Da
• NCBI Official Full Name: FasL isoform
• NCBI Official Synonym Full Names: Fas ligand (TNF superfamily, member 6)
• NCBI Official Symbol: Fasl
• NCBI Official Synonym Symbols: gld; CD178; CD95L; Fas-L; Faslg; CD95-L; Tnfsf6; APT1LG1
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 6; CD95 ligand; Fas antigen ligand; generalized lymphoproliferative disease
• UniProt Protein Name: FasL isoform
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: Fasl
• UniProt Entry Name: Q99PH8_MOUSE

Uniprot Description

FasL: Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. Homotrimer (Probable). Interacts with ARHGAP9, BAIAP2L1, BTK, CACNB3, CACNB4, CRK, DLG2, DNMBP, DOCK4, EPS8L3, FGR, FYB, FYN, HCK, ITK, ITSN2, KALRN, LYN, MACC1, MIA, MPP4, MYO15A, NCF1, NCK1, NCK2, NCKIPSD, OSTF1, PIK3R1, PSTPIP1, RIMBP3C, SAMSN1, SH3GL3, SH3PXD2B, SH3PXD2A, SH3RF2, SKAP2, SNX33, SNX9, SORBS3, SPTA1, SRC, SRGAP1, SRGAP2, SRGAP3, TEC, TJP3 and YES1. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Cytokine; Membrane protein, integral

Cellular Component: extracellular space; lysosome; cytoplasmic membrane-bound vesicle; extracellular region; integral to membrane; caveola; lipid raft; membrane; perinuclear region of cytoplasm; plasma membrane; cytoplasmic vesicle; nucleus; external side of plasma membrane

Molecular Function: death receptor binding; cytokine activity; tumor necrosis factor receptor binding

Biological Process: caspase activation; transcription, DNA-dependent; apoptosis; positive regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; cellular chloride ion homeostasis; endosomal lumen acidification; inflammatory cell apoptosis; negative regulation of angiogenesis; induction of apoptosis via death domain receptors; regulation of transcription, DNA-dependent; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of neuron apoptosis; positive regulation of cell proliferation; immune response; retinal cell programmed cell death