Human Fas ligand (TNF superfamily, member 6) ELISA Kit | FASLG elisa kit

Human Factor-related Apoptosis ligand, FASL ELISA Kit

Cat. Num. AAA704433

• Reactivity: Human
• Synonyms: Fas ligand (TNF superfamily; member 6); Human Factor-related Apoptosis ligand; FASL ELISA Kit; APT1LG1; CD178; CD95L; FASL; TNFSF6; CD95 ligand; apoptosis (APO-1) antigen ligand 1; fas ligand; tumor necrosis factor (ligand) superfamily; member 6; FASLG elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human FASL. No significant cross-reactivity or interference between human FASL and analogues was observed.
• Sequence Length: 281

• Samples: Serum, plasma, cell culture supernates
• Assay Type: Sandwich
• Detection Range: 31.25 pg/ml -2000 pg/ml.
• Sensitivity: The minimum detectable dose of human FASL is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FASLG elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FASL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FASL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FASL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FASL bound in the initial step. The color development is stopped and the intensity of the color is measured.