Sheep Interleukin 12, IL-12 ELISA Kit | IL-12 elisa kit

Sheep Interleukin 12, IL-12 ELISA Kit

Cat. Num. AAA700980

• Reactivity: Sheep
• Synonyms: Interleukin 12; IL-12; Sheep Interleukin 12; IL-12 ELISA Kit; IL-12 elisa kit

• Reactivity: Sheep
• Specificity: This assay has high sensitivity and excellent specificity for detection of sheep IL-12. No significant cross-reactivity or interference between sheep IL-12 and analogues was observed.
• Sequence Length: 862

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 31.25 pg/ml-2000 pg/ml.
• Sensitivity: The minimum detectable dose of sheep IL-12 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-12 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-12 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-12 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 1685028
• NCBI GeneID: 3595
• UniProt Accession #: Q99665; Q2M3V3; B1AN98; B7ZKL9; F5H7L6
• Molecular Weight: 87,484 Da
• NCBI Official Full Name: Il-12 receptor beta2
• NCBI Official Synonym Full Names: interleukin 12 receptor, beta 2
• NCBI Official Symbol: IL12RB2
• NCBI Protein Information: interleukin-12 receptor subunit beta-2; IL-12R subunit beta-2; IL-12 receptor subunit beta-2; interleukin-12 receptor beta-2 chain
• UniProt Protein Name: Interleukin-12 receptor subunit beta-2
• UniProt Gene Name: IL12RB2
• UniProt Synonym Gene Names: IL-12 receptor subunit beta-2; IL-12R subunit beta-2; IL-12R-beta-2; IL-12RB2
• UniProt Entry Name: I12R2_HUMAN

NCBI Description

The protein encoded by this gene is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. The coexpression of this and IL12RB1 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The expression of this gene is up-regulated by interferon gamma in Th1 cells, and plays a role in Th1 cell differentiation. The up-regulation of this gene is found to be associated with a number of infectious diseases, such as Crohn's disease and leprosy, which is thought to contribute to the inflammatory response and host defense. Several transcript variants encoding different isoforms and non-protein coding transcripts have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

Function: Receptor for interleukin-12. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. Promotes the proliferation of T-cells as well as NK cells. Induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production.