Typical Testing Data/Standard Curve (for reference only)
Bovine Angiotensin II Receptor 2 ELISA Kit | AT2R elisa kit
Bovine Angiotensin II Receptor 2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: AT2R ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AT2R antibody and an AT2R-HRP conjugate. The assay sample and buffer are incubated together with AT2R-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AT2R concentration since AT2R from samples and AT2R-HRP conjugate compete for the anti-AT2R antibody binding site. Since the number of sites is limited, as more sites are occupied by AT2R from the sample, fewer sites are left to bind AT2R-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AT2R concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the G-protein coupled receptor 1 family, and functions as a receptor for angiotensin II. It is an intergral membrane protein that is highly expressed in fetus, but scantily in adult tissues, except brain, adrenal medulla, and atretic ovary. This receptor has been shown to mediate programmed cell death and this apoptotic function may play an important role in developmental biology and pathophysiology. Mutations in this gene are been associated with X-linked mental retardation. [provided by RefSeq, Jan 2010]
Uniprot Description
AT2: Receptor for angiotensin II. Cooperates with MTUS1 to inhibit ERK2 activation and cell proliferation. Defects in AGTR2 are the cause of mental retardation X- linked type 88 (MRX88). Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Belongs to the G-protein coupled receptor 1 family.
Protein type: Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1
Chromosomal Location of Human Ortholog: Xq22-q23
Cellular Component: perinuclear region of cytoplasm; integral to plasma membrane; plasma membrane; extracellular region
Molecular Function: receptor antagonist activity; protein binding; angiotensin type II receptor activity; peptide hormone binding; transcription factor binding
Biological Process: positive regulation of phosphoprotein phosphatase activity; negative regulation of icosanoid secretion; regulation of transcription factor import into nucleus; positive regulation of nitric oxide biosynthetic process; negative regulation of heart rate; negative regulation of nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; renin-angiotensin regulation of aldosterone production; dopamine biosynthetic process; positive regulation of vasodilation; angiotensin mediated vasodilation involved in regulation of systemic arterial blood pressure; cerebellar cortex development; cell surface receptor linked signal transduction; regulation of systemic arterial blood pressure by circulatory renin-angiotensin; regulation of blood pressure; positive regulation of cell proliferation; inflammatory response; cellular sodium ion homeostasis; positive regulation of nitric-oxide synthase activity; response to organic nitrogen; negative regulation of blood vessel endothelial cell migration; brain renin-angiotensin system; G-protein coupled receptor protein signaling pathway; negative regulation of fibroblast proliferation; blood vessel remodeling; G-protein signaling, coupled to cGMP nucleotide second messenger; brain development; negative regulation of cell growth; nitric oxide mediated signal transduction; positive regulation of cytokine secretion
Research Articles on AT2R
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Product Notes
The Bovine AT2R agtr2 (Catalog #AAA746823) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA746823 ELISA Kit recognizes Bovine AT2R. It is sometimes possible for the material contained within the vial of "Angiotensin II Receptor 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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