Human ERBB receptor feedback inhibitor 1 ELISA Kit | ERRFI1 elisa kit

Human ERBB receptor feedback inhibitor 1, ERRFI1 ELISA Kit

Cat. Num. AAA907410

• Gene Names: ERRFI1; MIG6; RALT; MIG-6; GENE-33
• Reactivity: Human
• Synonyms: ERBB receptor feedback inhibitor 1; Human ERBB receptor feedback inhibitor 1; ERRFI1 ELISA Kit; Human ERBB receptor feedback inhibitor 1 (ERRFI1) ELISA kit; CTA-215D11.2; GENE-33; MIG-6; MIG6; RALT; mitogen-inducible gene 6; mitogen-inducible gene 6 protein; receptor-associated late transducer; ERRFI1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human ERRFI1. No significant cross-reactivity or interference between human ERRFI1 and analogues was observed.
• Sequence Length: 462

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 31.25 pg/ml-2000 pg/ml.
• Sensitivity: The minimum detectable dose of human ERRFI1 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ERRFI1 elisa kit

Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ERRFI1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ERRFI1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ERRFI1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ERRFI1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 10047086
• NCBI GeneID: 54206
• NCBI Accession #: NP_061821.1
• NCBI GenBank Nucleotide #: NM_018948.3
• UniProt Accession #: Q9UJM3; Q9NTG9; Q9UD05; B2RDX9
• Molecular Weight: 50,560 Da
• NCBI Official Full Name: ERBB receptor feedback inhibitor 1
• NCBI Official Synonym Full Names: ERBB receptor feedback inhibitor 1
• NCBI Official Symbol: ERRFI1
• NCBI Official Synonym Symbols: MIG6; RALT; MIG-6; GENE-33
• NCBI Protein Information: ERBB receptor feedback inhibitor 1; mitogen-inducible gene 6 protein; receptor-associated late transducer
• UniProt Protein Name: ERBB receptor feedback inhibitor 1
• Protein Family: ERBB receptor feedback inhibitor
• UniProt Gene Name: ERRFI1
• UniProt Synonym Gene Names: MIG6; MIG-6
• UniProt Entry Name: ERRFI_HUMAN

NCBI Description

ERRFI1 is a cytoplasmic protein whose expression is upregulated with cell growth (Wick et al., 1995 [PubMed 7641805]). It shares significant homology with the protein product of rat gene-33, which is induced during cell stress and mediates cell signaling (Makkinje et al., 2000 [PubMed 10749885]; Fiorentino et al., 2000 [PubMed 11003669]).[supplied by OMIM, Mar 2008]

Uniprot Description

MIG-6: Negative regulator of EGFR signaling in skin morphogenesis. Acts as a negative regulator for several EGFR family members, including ERBB2, ERBB3 and ERBB4. Inhibits EGFR catalytic activity by interfering with its dimerization. Inhibits autophosphorylation of EGFR, ERBB2 and ERBB4. Important for normal keratinocyte proliferation and differentiation. Plays a role in modulating the response to steroid hormones in the uterus. Required for normal response to progesterone in the uterus and for fertility. Mediates epithelial estrogen responses in the uterus by regulating ESR1 levels and activation. Important for regulation of endometrium cell proliferation. Important for normal prenatal and perinatal lung development. Interacts with ERBB2. Interacts with EGFR. Levels are very low in quiescent cells. Up-regulated by mitogens. Belongs to the MIG6 family.

Protein type: Tumor suppressor; Inhibitor

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: extrinsic to internal side of plasma membrane; cytoplasm; nucleus

Molecular Function: protein binding; protein kinase binding

Biological Process: negative regulation of epidermal growth factor receptor activity; skin morphogenesis; response to stress; negative regulation of protein amino acid autophosphorylation; regulation of keratinocyte differentiation; alveolus development