Rabbit anti-Human, Mouse 5-HT-2C Polyclonal Antibody | anti-5-HT-2C antibody

5-HT-2C Antibody

Cat. Num. AAA9406170

• Gene Names: HTR2C; HTR1C; 5-HT1C; 5-HT2C; 5HTR2C; 5-HTR2C
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: 5-HT-2C; Polyclonal Antibody; 5-HT-2C Antibody; 5-hydroxytryptamine 2C receptor; 5H2C; 5HT-1C; 5HT1C; 5HT2C; anti-5-HT-2C antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total 5-HT-2C protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 458

• Applicable Applications for anti-5-HT-2C antibody: Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Immunohistochemistry: 1:50~1:100; Immunofluorescence: 1:100~1:500
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from internal of human 5-HT-2C.
• Target Name: 5-HT-2C
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human brain tissue, using 5-HT-2C antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cells, using 5-HT-2C antibody.)

Related Product Information for anti-5-HT-2C antibody

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including ergot alkaloid derivatives, 1-2,5,-dimethoxy-4-iodophenyl-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and down-stream signaling cascades and promotes the release of Ca2+ ions from intracellular stores. Regulates neuronal activity via the activation of short transient receptor potential calcium channels in the brain, and thereby modulates the activation of pro-opiomelacortin neurons and the release of CRH that then regulates the release of corticosterone. Plays a role in the regulation of appetite and eating behavior, responses to anxiogenic stimuli and stress. Plays a role in insulin sensitivity and glucose homeostasis.Saltzman A.G., Biochem. Biophys. Res. Commun. 181:1469-1478(1991).Stam N.J., Eur. J. Pharmacol. 269:339-348(1994).Xie E., Genomics 35:551-561(1996).

Product Categories/Family for anti-5-HT-2C antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4504541
• NCBI GeneID: 3358
• NCBI Accession #: NP_000859.1
• NCBI GenBank Nucleotide #: NM_000868.3
• UniProt Accession #: P28335; Q5VUF8; Q9NP28; B1AMW4
• Molecular Weight: 28,055 Da
• NCBI Official Full Name: 5-hydroxytryptamine receptor 2C isoform a
• NCBI Official Synonym Full Names: 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled
• NCBI Official Symbol: HTR2C
• NCBI Official Synonym Symbols: HTR1C; 5-HT1C; 5-HT2C; 5HTR2C; 5-HTR2C
• NCBI Protein Information: 5-hydroxytryptamine receptor 2C
• UniProt Protein Name: 5-hydroxytryptamine receptor 2C
• UniProt Gene Name: HTR2C
• UniProt Synonym Gene Names: HTR1C; 5-HT-2C; 5-HT2C; 5-HTR2C; 5-HT-1C; 5-HT1C
• UniProt Entry Name: 5HT2C_HUMAN

NCBI Description

This gene encodes a seven-transmembrane G-protein-coupled receptor. The encoded protein responds to signaling through the neurotransmitter serotonin. The mRNA of this gene is subject to multiple RNA editing events, where adenosine residues encoded by the genome are converted to inosines. RNA editing is predicted to alter the structure of the second intracellular loop, thereby generating alternate protein forms with decreased ability to interact with G proteins. Abnormalities in RNA editing of this gene have been detected in victims of suicide that suffer from depression. In addition, naturally-occuring variation in the promoter and 5' non-coding and coding regions of this gene may show statistically-significant association with mental illness and behavioral disorders. Alternative splicing results in multiple different transcript variants. [provided by RefSeq, Jan 2015]

Uniprot Description

5-HT(2C): a G-protein coupled receptor. One of several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. Mediates its action by activating a phosphatidylinositol-calcium second messenger system.

Protein type: Membrane protein, integral; GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: Xq24

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: serotonin binding; serotonin receptor activity; drug binding; 5-HT2 receptor activity

Biological Process: response to drug; cGMP biosynthetic process; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); behavioral fear response; serotonin receptor, phospholipase C activating pathway; regulation of corticotropin-releasing hormone secretion; locomotory behavior; positive regulation of phosphatidylinositol biosynthetic process; cellular calcium ion homeostasis; synaptic transmission; regulation of neurological process; release of sequestered calcium ion into cytosol; regulation of appetite; positive regulation of fat cell differentiation; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); feeding behavior; serotonin receptor signaling pathway