NEGR1 recombinant protein
Recombinant Human NEGR1 Protein (His tag)
Gene Names
NEGR1; Ntra; KILON; IGLON4; DMML2433
Purity
> 95 % as determined by SDS-PAGE
Synonyms
NEGR1; Recombinant Human NEGR1 Protein (His tag); UNQ2433; DMML2433; IGLON4; KILON; MGC46680; Ntra; NEGR1 recombinant protein
Host
Human Cells
Purity/Purification
> 95 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01% tween-80.
Sequence
Met 1-Gly 324
Tag
C-His
Expression Host
HEK293 Cells
Endotoxin
< 1.0 EU per ug as determined by the LAL method
Reconstitution
We recommend that the vial be centrifuged to opening to bring the contents to the bottom. Do not mix by vortex. It is recommended that sterile water(400ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV-Vis.
Preparation and Storage
Lyophilized proteins are stable when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C. Aliquots of reconstituted samples are stable at < -20°C. This product is provided as lyophilized powder which is shipped at 4°C.
SDS-PAGE
Related Product Information for NEGR1 recombinant protein
Background: Neuronal Growth Regulator 1, NEGR1, also known as neurotractin, or KILON, which belongs to the immunoglobulin superfamily, IgLON family. This GPI-linked cell surface glycoprotein NEGR1 is composed of three Ig-like domains and belongs to the IgLON subgroup of neural IgSF members. It is expressed in two isoforms with apparent molecular masses of 50 and 37 kD, termed L-form and S-form, respectively. NEGR1/Neurotractin participates in the regulation of neurite outgrowth in the developing brain, and is expressed on neurites of primary hippocampal neurons. Neurotractin/KILON is a trans-neural growth-promoting factor for outgrowing axons following hippocampal denervation. KILON (kindred of IgLON) and opioid-binding cell adhesion molecule belong to the IgLON subgroup of immunoglobulin superfamily together with the limbic system-associated membrane protein and neurotrimin. The alteration of modulatory function of KILON/NEGR1 for the number of dendritic synapses concomitant with changes in its localization and detergent solubility during neuronal culture development. In addition to its reported role in the brain, NEGR1 is also expressed in subcutaneous adipose tissue and acts as a central 'hub' in an obesity-related transcript network.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Molecular Mass: 32.9 kDa
AP Molecular Mass: 45-48 kDa
AP Molecular Mass: 45-48 kDa
NCBI Official Full Name
neuronal growth regulator 1
NCBI Official Synonym Full Names
neuronal growth regulator 1
NCBI Official Symbol
NEGR1
NCBI Official Synonym Symbols
Ntra; KILON; IGLON4; DMML2433
NCBI Protein Information
neuronal growth regulator 1
UniProt Protein Name
Neuronal growth regulator 1
Protein Family
UniProt Gene Name
NEGR1
UniProt Synonym Gene Names
IGLON4
Uniprot Description
NEGR1: May be involved in cell-adhesion. May function as a trans-neural growth-promoting factor in regenerative axon sprouting in the mammalian brain. Belongs to the immunoglobulin superfamily. IgLON family.
Protein type: Membrane protein, GPI anchor
Chromosomal Location of Human Ortholog: 1p31.1
Molecular Function: protein binding
