Rabbit anti-Human FADD Polyclonal Antibody | anti-FADD antibody

FADD (Ab-191) Antibody

Cat. Num. AAA9412480

• Gene Names: Fadd; Mort1/FADD
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: FADD; Polyclonal Antibody; FADD (Ab-191) Antibody; Fas (TNFRSF6)-associated via death domain; FAS-associated death domain protein; FAS-associating death domain-containing protein; Fas-associating protein with death domain; anti-FADD antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total FADD protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 205

• Applicable Applications for anti-FADD antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500~1:3000
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from C-terminal of human FADD.
• Target Name: FADD
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from A549 cells (Lane 2), using FADD (Ab-191) antiobdy. The lane on the left is treated with synthesized peptide.)

Related Product Information for anti-FADD antibody

Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis By similarity. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling By similarity.Zhang J., Mol. Cell. Biol. 16:2756-2763(1996).Hsu H., Cell 84:299-308(1996).Carninci P., Science 309:1559-1563(2005)

Product Categories/Family for anti-FADD antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 6753812
• NCBI GeneID: 14082
• NCBI Accession #: NP_034305.1
• NCBI GenBank Nucleotide #: NM_010175.5
• UniProt Accession #: Q61160; Q3TC37; Q61082
• Molecular Weight: 22,960 Da
• NCBI Official Full Name: FAS-associated death domain protein
• NCBI Official Synonym Full Names: Fas (TNFRSF6)-associated via death domain
• NCBI Official Symbol: Fadd
• NCBI Official Synonym Symbols: Mort1/FADD
• NCBI Protein Information: FAS-associated death domain protein
• UniProt Protein Name: FAS-associated death domain protein
• Protein Family: Fas-associated death domain protein
• UniProt Gene Name: Fadd
• UniProt Synonym Gene Names: Mort1
• UniProt Entry Name: FADD_MOUSE

Uniprot Description

FADD: an adaptor molecule that mediates apoptosis. Recruited through its C-terminal death domain by Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TRAIL-receptor, participating in the death signaling initiated by these receptors. Interaction with the receptors unmasks the N-terminal effector domain of this protein, which recruits caspase-8, and thereby activates the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.

Protein type: Adaptor/scaffold; Apoptosis

Cellular Component: neuron projection; protein complex; cytoplasm; CD95 death-inducing signaling complex; cytosol; lipid raft

Molecular Function: identical protein binding; protein binding; protease binding; death receptor binding; protein complex binding; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding

Biological Process: spleen development; cardiac muscle development; thymus development; positive regulation of apoptosis; positive regulation of proteolysis; protein heterooligomerization; positive regulation of T cell mediated cytotoxicity; apoptosis; immune system process; T cell differentiation in the thymus; signal transduction; positive regulation of activated T cell proliferation; positive regulation of tumor necrosis factor production; lymph node development; regulation of apoptosis; positive regulation of interleukin-8 production; positive regulation of interferon-gamma production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; T cell homeostasis; positive regulation of macrophage differentiation; defense response to virus; positive regulation of adaptive immune response; T cell differentiation