Testing Data
JAM-A/Junctional Adhesion Molecule A ELISA Pair Set | F11R elisa pair set
JAM-A/Junctional Adhesion Molecule A Matched ELISA Antibody Pair Set, Human
Synonyms
JAM-A/Junctional Adhesion Molecule A; JAM-A/Junctional Adhesion Molecule A Matched ELISA Antibody Pair Set; Human; Human Junctional Adhesion Molecule A/JAM-A ELISA Pair Set; F11 receptor; CD321 Matched ELISA Antibody Pair Set; JAM Matched ELISA Antibody Pair Set; JAM1 Matched ELISA Antibody Pair Set; JAMA Matched ELISA Antibody Pair Set; JCAM Matched ELISA Antibody Pair Set; KAT Matched ELISA Antibody Pair Set; PAM-1 Matched ELISA Antibody Pair Set; F11R elisa pair set
Specificity
Quantitative determination of Human JAM-A/Junctional Adhesion Molecule A
Assay Type
Solid Phase Sandwich ELISA
Detection Range
7.8-500pg/mL
Sensitivity
7.8pg/ml
Materials Provided
1. Capture Antibody 0.5 mg/mL of mouse anti-Human Junctional Adhesion Molecule A/JAM-A monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating.
2. Detection Antibody 0.1 mg/mL of mouse anti-Human Junctional Adhesion Molecule A/JAM-A monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.4ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 10 ng of recombinant Human Junctional Adhesion Molecule A/JAM-A. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 500 pg/mL is recommended.
2. Detection Antibody 0.1 mg/mL of mouse anti-Human Junctional Adhesion Molecule A/JAM-A monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.4ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 10 ng of recombinant Human Junctional Adhesion Molecule A/JAM-A. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 500 pg/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for F11R elisa pair set
Junctional adhesion molecule-A (JAM-A), also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321), is a transmembrane protein expressed at tight junctions of epithelial and endothelial cells, as well as on circulating leukocytes. JAM-A protein serves as a serotype-independent receptor for mammalian orthoreoviruses (reoviruses). It is also a ligand for the integrin LFA1, involves in leukocyte transmigration. As a cell adhesion molecule of the immunoglobulin superfamily, JAM-A protein involves in platelet adhesion, secretion and aggregation, and plays a crucial role in inflammatory thrombosis and atherosclerosis. In addition, it may be a potential therapeutic target for breast cancer.
This JAM-A/Junctional Adhesion Molecule A Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human JAM-A/Junctional Adhesion Molecule A. It contains Human JAM-A/Junctional Adhesion Molecule A capture antibody, Human JAM-A/Junctional Adhesion Molecule A detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human JAM-A/Junctional Adhesion Molecule A protein.
This JAM-A/Junctional Adhesion Molecule A Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human JAM-A/Junctional Adhesion Molecule A. It contains Human JAM-A/Junctional Adhesion Molecule A capture antibody, Human JAM-A/Junctional Adhesion Molecule A detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human JAM-A/Junctional Adhesion Molecule A protein.
References
Guglielmi KM, et al. (2007) Reovirus binding determinants in junctional adhesion molecule-A. J Biol Chem. 282(24): 17930-40.Yeung D, et al. (2008) Decreased junctional adhesion molecule-A expression during blood-brain barrier breakdown. Acta Neuropathol. 115(6): 635-42.Ong KL, et al. (2009) Elevated plasma level of soluble F11 receptor/junctional adhesion molecule-A (F11R/JAM-A) in hypertension. Am J Hypertens. 22(5): 500-5.
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Product Notes
The F11R (Catalog #AAA8118552) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "JAM-A/Junctional Adhesion Molecule A, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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