Western Blot (WB)
(Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.)
Chicken ATP6J Polyclonal Antibody | anti-ATP6V1G1 antibody
ATP6J (ATPase, H+ Transporting, Lysosomal, V1 Subunit G Isoform 1, Member J)
Gene Names
ATP6V1G1; ATP6J
Reactivity
Bovine, Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
ATP6J; Polyclonal Antibody; ATP6J (ATPase; H+ Transporting; Lysosomal; V1 Subunit G Isoform 1; Member J); Anti -ATP6J (ATPase; anti-ATP6V1G1 antibody
Host
Chicken
Reactivity
Bovine, Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgY
Specificity
Recognizes human ATP6J. Species Crossreactivity: Mouse, rat and bovine.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Glycerol free.
Applicable Applications for anti-ATP6V1G1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1:1000-1:2000
Dilution: Western Blot: 1:1000-1:2000
Immunogen
Synthetic peptide corresponding to aa1-118
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.)
Western Blot (WB)
(Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.)
Product Categories/Family for anti-ATP6V1G1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
13,682 Da
NCBI Official Full Name
ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G1
NCBI Official Synonym Full Names
ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G1
NCBI Official Symbol
ATP6V1G1
NCBI Official Synonym Symbols
ATP6J
NCBI Protein Information
V-type proton ATPase subunit G 1; V-ATPase subunit G 1; vacuolar H+ ATPase G1; V-ATPase 13 kDa subunit 1; vacuolar proton pump subunit G 1; vacuolar proton pump subunit M16; ATPase, H+ transporting, lysosomal (vacuolar proton pump), member J
UniProt Protein Name
V-type proton ATPase subunit G 1
Protein Family
UniProt Gene Name
ATP6V1G1
UniProt Synonym Gene Names
ATP6G; ATP6G1; V-ATPase subunit G 1
UniProt Entry Name
VATG1_BOVIN
Uniprot Description
Function: Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
Subunit structure: V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d).
Tissue specificity: Brain, heart, kidney and spleen. Ref.3
Sequence similarities: Belongs to the V-ATPase G subunit family.
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Product Notes
The ATP6V1G1 atp6v1g1 (Catalog #AAA615058) is an Antibody produced from Chicken and is intended for research purposes only. The product is available for immediate purchase. The ATP6J (ATPase, H+ Transporting, Lysosomal, V1 Subunit G Isoform 1, Member J) reacts with Bovine, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATP6J can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Western Blot: 1:1000-1:2000. Researchers should empirically determine the suitability of the ATP6V1G1 atp6v1g1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATP6J, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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