Rat Kidney Injury Molecule 1 (Kim1) RTU ELISA Kit | Kim1 rtu elisa kit
Rat Kidney Injury Molecule 1 (Kim1) ELISA Kit
Gene Names
Havcr1; Kim1; KIM-1
Reactivity
Rat
Synonyms
Kidney Injury Molecule 1 (Kim1); Rat Kidney Injury Molecule 1 (Kim1) ELISA Kit; HAVCR1; TIM1; TIMD1; HAVCR; Hepatitis A Virus Cellular Receptor 1; T Cell Immunoglobulin And Mucin Domain-Containing Protein 1; T-cell immunoglobulin mucin receptor 1; Kim1 rtu elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Kim1. No significant cross-reactivity or interference between Kim1 and analogues was observed.
Sequence Length
307
Samples
Rat tissue homogenates, urine, cell culture supernates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
62.5-4000pg/mL
Sensitivity
< 22.6pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kim1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kim1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Kim1 rtu elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of Kim1 in rat tissue homogenates, urine, cell culture supernates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kim1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to Kim1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kim1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Kim1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kim1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to Kim1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kim1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Kim1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,964 Da
NCBI Official Full Name
hepatitis A virus cellular receptor 1 homolog
NCBI Official Synonym Full Names
hepatitis A virus cellular receptor 1
NCBI Official Symbol
Havcr1
NCBI Official Synonym Symbols
Kim1; KIM-1
NCBI Protein Information
hepatitis A virus cellular receptor 1 homolog
UniProt Protein Name
Hepatitis A virus cellular receptor 1 homolog
UniProt Gene Name
Havcr1
UniProt Synonym Gene Names
Kim1; HAVcr-1; KIM-1; TIMD-1
NCBI Description
may be a member of the mucosal addressin cell adhesion molecule family; may be involved in restoration of the morphological integrity and function to postischemic kidney [RGD, Feb 2006]
Uniprot Description
May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4. May play a role in kidney injury and repair ().
