super leptin antagonist Active Protein | SOLA active protein
Ovine super leptin antagonist (mutant D23/L39A/D40A/F41A)
Purity
The purity of recombinant super active ovine leptin antagonist is greater than 98.0% as determined by:(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
Synonyms
super leptin antagonist; Ovine super leptin antagonist (mutant D23/L39A/D40A/F41A); Ovine super-active leptin antagonist (SOLA); SOLA active protein
Host
Escherichia Coli
Purity/Purification
The purity of recombinant super active ovine leptin antagonist is greater than 98.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
Form/Format
White lyophilized (freeze-dried) powder.
Sequence
The sequence of the first five N-terminal amino acids of recombinant super active ovine leptin antagonist was determined and was found to be Ala-Val-Pro-Ile-Arg
Sequence Length
371
Solubility
It is recommended to reconstitute the lyophilized recombinant super active ovine leptin antagonist in sterile water or sterile 0.4% NaHCO3 adjusted to pH 10, not less than 100ug/ml, which can then be further diluted with other aqueous solutions.
Protein Content
Protein quantitation of recombinant super active ovine leptin antagonist was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.200 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Dimers and Aggregates
The purified recombinant super active ovine leptin antagonist (16K) consists of > 95% monomers as determined by gel-filtration chromatography.
Endotoxin
The endotoxin content of recombinant super active ovine leptin antagonist is less than 0.02 ng/ug (0.2 EU/ug) of ovine leptin antagonist.
Formulation
The recombinant super active ovine leptin antagonist was lyophilized from a concentrated (1 mg/ml) solution with 0.003mM NaHCO3 adjusted to pH 10.
Biological Activity
recombinant super active ovine leptin antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. It also inhibits various leptin effects in several in vitro bioassays. The inhibitory activity of recombinant super active ovine leptin antagonist was increased 14 to 60 fold as measured by various criteria such as binding properties to human leptin binding domain (hLBD) and in vitro bioassays as compared to recombinant ovine leptin antagonist.
Preparation and Storage
Lyophilized recombinant super active ovine leptin antagonist although stable at room temperature for several weeks, should be stored desiccated below -20 degree C. Upon reconstitution of recombinant super active ovine leptin antagonist at > 0.1 mg/ml and up to 2 mg/ml and filter sterilization recombinant super active ovine leptin antagonist can be stored at 4 degree C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration of recombinant super active ovine leptin antagonist addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.
Related Product Information for SOLA active protein
Recombinant mouse leptin, one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, was mutated, resulting in D23L/L39A/D40A/F41A mutant termed recombinant super active ovine leptin antagonist that was purified by proprietary chromatographic techniques.
Product Categories/Family for SOLA active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,914 Da
NCBI Official Full Name
SolA
UniProt Protein Name
N-methyl-L-tryptophan oxidase
Protein Family
UniProt Gene Name
solA
UniProt Synonym Gene Names
MTOX
UniProt Entry Name
D4GC14_PANAM
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Product Notes
The SOLA sola (Catalog #AAA400083) is an Active Protein produced from Escherichia Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first five N-terminal amino acids of recombinan t super active ovine leptin antagonist was determined and was found to be Ala-Val-Pr o-Ile-Arg. It is sometimes possible for the material contained within the vial of "super leptin antagonist, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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