Rabbit MFNG Polyclonal Antibody | anti-MFNG antibody

MFNG Polyclonal Antibody

Cat. Num. AAA3007513

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Synonyms: MFNG; Polyclonal Antibody; MFNG Polyclonal Antibody; Beta-1; 3-N-acetylglucosaminyltransferase manic fringe; O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase; anti-MFNG antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MFNG polyclonal antibody detects endogenous levels of MFNG protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 307

• Applicable Applications for anti-MFNG antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 66-110 of Human MFNG
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MFNG polyclonal antibody at 1:500 dilutionLane1:SGC7901 whole cell lysate (40ug) Lane2:Hct116 whole cell lysate (40ug) Lane3:The Spleen tissue lysate of Mouse (40ug) Lane4:The Spleen tissue lysate of Rat (40ug) Lane5:L02 whole cell lysate (40ug))

Related Product Information for anti-MFNG antibody

Glycosyltransferase involved in the elongation of O-linked ligands to activate Notch signaling. Possesses fucose-specific beta-1, 3-N-acetylglucosaminyltransferase activity.

NCBI and Uniprot Product Information

• NCBI GI #: 261824031
• NCBI GeneID: 4242
• NCBI Accession #: NP_001159815.1
• NCBI GenBank Nucleotide #: NP_001159815.1
• UniProt Accession #: O00587
• Molecular Weight: ~ 36kDa
• NCBI Official Full Name: beta-1,3-N-acetylglucosaminyltransferase manic fringe isoform 2
• NCBI Official Synonym Full Names: MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
• NCBI Official Symbol: MFNG
• NCBI Protein Information: beta-1,3-N-acetylglucosaminyltransferase manic fringe
• UniProt Protein Name: Beta-1,3-N-acetylglucosaminyltransferase manic fringe
• Protein Family: Beta-1,3-N-acetylglucosaminyltransferase manic fringe
• UniProt Gene Name: MFNG
• UniProt Entry Name: MFNG_HUMAN

NCBI Description

This gene is a member of the glycosyltransferase 31 gene family. Members of this gene family, which also includes the LFNG (GeneID: 3955) and RFNG (GeneID: 5986) genes, encode evolutionarily conserved glycosyltransferases that act in the Notch signaling pathway to define boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, these proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. The protein encoded by this gene may control Notch signaling in claudin-low breast cancer. [provided by RefSeq, May 2018]

Uniprot Description

MFNG: Glycosyltransferase involved in the elongation of O- linked ligands to activate Notch signaling. Possesses fucose- specific beta-1,3-N-acetylglucosaminyltransferase activity. Belongs to the glycosyltransferase 31 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; Membrane protein, integral; EC 2.4.1.222

Chromosomal Location of Human Ortholog: 22q12

Cellular Component: extracellular space; integral to Golgi membrane

Molecular Function: metal ion binding; O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity

Biological Process: positive regulation of protein binding; metabolic process; pattern specification process; positive regulation of Notch signaling pathway