Cytidine Deaminase Active Protein | CDA active protein

Recombinant Human Cytidine Deaminase

Cat. Num. AAA144281

• Gene Names: CDA; CDD
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Cytidine Deaminase; Recombinant Human Cytidine Deaminase; Cytidine deaminase; Cytidine aminohydrolase; CDA; CDD.; CDA active protein

• Host: Escherichia Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: The CDA solution (0.5 mg/ml) contains 20 mM Tris-HCl Buffer (pH 8.0), 1 mM DTT, 2mM EDTA, 100 mM NaCl and 40% Glycerol.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MAQKRPACTL KPECVQQLLV CSQEAKQSAY CPYSHFPVGA ALLTQEGRIF KGCNIENACY PLGICAERTA IQKAVSEGYK DFRAIAIASD MQDDFISPCG ACRQVMREFG TNWPVYMTKP DGTYIVMTVQ ELLPSSFGPE DLQKTQ
• Sequence Length: 146

• Biological Activity: Specific activity :> 3.5unit/mg. One unit of activity was defined as the amount required to deaminate 1.0 umole of cytidine per min at pH 7.5 at 25 degree C.
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks.Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for CDA active protein

Description: CDA Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 166 amino acids (1-146 a.a.) and having a molecular mass of 18.3kDa. The CDA is purified by proprietary chromatographic techniques.

Introduction: Cytidine deaminase (CDA) is an enzyme that scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. CDA is one of several deaminases responsible for maintaining the cellular pyrimidine pool. CDA also catalyzes the deamination of chemotherapeutic cytosine nucleoside analogs such as Ara-C and 5-azacytidine, which results in the loss of their cytotoxic and antitumor function. CDA can form homotetramers and is generally expressed in granulocytes. Mutations in the CDA gene are linked to decreased sensitivity to the cytosine nucleoside analogue cytosine arabinoside used in the treatment of certain childhood leukemias.

Product Categories/Family for CDA active protein

Enzymes; Deaminase

NCBI and Uniprot Product Information

• NCBI GI #: 11386157
• NCBI GeneID: 978
• NCBI Accession #: NP_001776.1
• NCBI GenBank Nucleotide #: NM_001785.2
• UniProt Accession #: P32320
• Molecular Weight: 16,185 Da
• NCBI Official Full Name: cytidine deaminase
• NCBI Official Synonym Full Names: cytidine deaminase
• NCBI Official Symbol: CDA
• NCBI Official Synonym Symbols: CDD
• NCBI Protein Information: cytidine deaminase; cytidine aminohydrolase; cytosine nucleoside deaminase; small cytidine deaminase
• UniProt Protein Name: Cytidine deaminase
• Protein Family: Colicin
• UniProt Gene Name: CDA
• UniProt Synonym Gene Names: CDD
• UniProt Entry Name: CDD_HUMAN

NCBI Description

This gene encodes an enzyme involved in pyrimidine salvaging. The encoded protein forms a homotetramer that catalyzes the irreversible hydrolytic deamination of cytidine and deoxycytidine to uridine and deoxyuridine, respectively. It is one of several deaminases responsible for maintaining the cellular pyrimidine pool. Mutations in this gene are associated with decreased sensitivity to the cytosine nucleoside analogue cytosine arabinoside used in the treatment of certain childhood leukemias. [provided by RefSeq, Jul 2008]

Uniprot Description

CDA: This enzyme scavenge exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. Belongs to the cytidine and deoxycytidylate deaminase family.

Protein type: Nucleotide Metabolism - pyrimidine; Hydrolase; EC 3.5.4.5; Xenobiotic Metabolism - drug metabolism - other enzymes

Chromosomal Location of Human Ortholog: 1p36.2-p35

Cellular Component: extracellular region; cytosol

Molecular Function: protein homodimerization activity; zinc ion binding; nucleoside binding; cytidine deaminase activity

Biological Process: cell surface receptor linked signal transduction; pyrimidine base metabolic process; cytosine metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleoside salvage; cytidine deamination; negative regulation of cell growth; pyrimidine salvage; negative regulation of nucleotide metabolic process; protein homotetramerization