Mouse CD48 Monoclonal Antibody | anti-CD48 antibody

Anti-Hu CD48 Pacific Blue

• Gene Names: CD48; BCM1; BLAST; hCD48; mCD48; BLAST1; SLAMF2; MEM-102
• Reactivity: Non-human primates, Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with Pacific Blue under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD48; Monoclonal Antibody; Anti-Hu CD48 Pacific Blue; BCM1; BLAST; BLAST1; SLAMF2; CD48 molecule; anti-CD48 antibody

• Host: Mouse
• Reactivity: Non-human primates, Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: MEM-102
• Specificity: The antibody MEM-102 reacts with CD48 (Blast-1), a 40-47kDa GPI-anchored extracellular membrane protein (immunoglobulin supergene family) widely expressed on hematopoietic cells; it is negative on granulocytes, platelets and erythrocytes.
• Purity/Purification: The purified antibody is conjugated with Pacific Blue under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Pacific Blue. Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-CD48 antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Immunogen: Raji human Burkitt's lymphoma cell line
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis (surface staining) of human peripheral blood with anti-CD48 (MEM-102) Pacific Blue™.)

Flow Cytometry (FC/FACS)

(Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD48 (MEM-102) Pacific Blue™ antibody (4 ul reagent / 100 ul of peripheral whole blood).)

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD48 (MEM-102) Pacific Blue™ antibody (4 ul reagent / 100 ul of peripheral whole blood).)

Related Product Information for anti-CD48 antibody

CD48 (Blast-1) belongs to the CD2 subset of the Ig superfamily, which includes CD2, CD2F-10, CD58, CD84, CD150, CD229, CD244 and others. These molecules bind to the same or another members of their family, thus mediate homotypic or heterotypic adhesion. CD48 is a GPI-anchored protein broadly expressed on hematopoietic cells and serves as a high affinity ligand for 2B4 and low affinity ligand for CD2. 2B4-CD48 interaction among NK cells and NK-T cells regulates cell proliferation. Signaling through CD48 results in eosinophil activation and CD48 expression is increased in several infectious diseases.

References

*Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989)., URL:,*Korinek V, Stefanova I, Angelisova P, Hilgert I, Horejsi V: The human leucocyte antigen CD48 (MEM-102) is closely related to the activation marker Blast-1. Immunogenetics. 1991;33(2):108-12., URL: https://www.ncbi.nlm.nih.gov/pubmed/1999350dopt=A,*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995)., URL:,*Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84., URL: https://www.ncbi.nlm.nih.gov/pubmed/17446208itool,*Angelisová P, Drbal K, Horejsí V, Cerný J: Association of CD10/neutral endopeptidase 24.11 with membrane microdomains rich in glycosylphosphatidylinositol-anchored proteins and Lyn kinase. Blood. 1999 Feb 15;93(4):1437-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/10075459itool,*Stulnig TM, Berger M, Sigmund T, Stockinger H, Horejsí V, Waldhäusl W: Signal transduction via glycosyl phosphatidylinositol-anchored proteins in T cells is inhibited by lowering cellular cholesterol. J Biol Chem. 1997 Aug 1;272(31):19242-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/9235917,*Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Bukovsky A, Horejsi V: Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol (Praha). 1989;35(5):289-97., URL: https://www.ncbi.nlm.nih.gov/pubmed/2534480dopt=A,*Schatzlmaier P, Supper V, Göschl L, Zwirzitz A, Eckerstorfer P, Ellmeier W, Huppa JB, Stockinger H: Rapid multiplex analysis of lipid raft components with single-cell resolution. Sci Signal. 2015 Sep 22;8(395):rs11, URL: http://www.ncbi.nlm.nih.gov/pubmed/26396269
*Assarsson E, Kambayashi T, Persson CM, Chambers BJ, Ljunggren HG: 2B4/CD48-mediated regulation of lymphocyte activation and Functional Application (FUNC)tion. J Immunol. 2005 Aug 15;175(4):2045-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/16081768ordin,*Mathew SO, Kumaresan PR, Lee JK, Huynh VT, Mathew PA: Mutational analysis of the human 2B4 (CD244)/CD48 interaction: Lys68 and Glu70 in the V domain of 2B4 are critical for CD48 binding and Functional Application (FUNC)tional activation of NK cells. J Immunol. 2005 Jul 15;175(2):1005-13., URL: https://www.ncbi.nlm.nih.gov/pubmed/16002700ordin,*Lee KM, Forman JP, McNerney ME, Stepp S, Kuppireddi S, Guzior D, Latchman YE, Sayegh MH, Yagita H, Park CK, Oh SB, Wülfing C, Schatzle J, Mathew PA, Sharpe AH, Kumar V: Requirement of homotypic NK-cell interactions through 2B4(CD244)/CD48 in the generation of NK effector Functional Application (FUNC)tions. Blood. 2006 Apr 15;107(8):3181-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/15905190ordin,*Munitz A, Bachelet I, Eliashar R, Khodoun M, Finkelman FD, Rothenberg ME, Levi-Schaffer F: CD48 is an allergen and IL-3-induced activation molecule on eosinophils. J Immunol. 2006 Jul 1;177(1):77-83., URL: https://www.ncbi.nlm.nih.gov/pubmed/16785501ordin

NCBI and Uniprot Product Information

• NCBI GI #: 114871
• NCBI GeneID: 962
• UniProt Accession #: P09326; Q5U055; Q8MGR0
• Molecular Weight: 19,429 Da
• NCBI Official Full Name: CD48 antigen
• NCBI Official Synonym Full Names: CD48 molecule
• NCBI Official Symbol: CD48
• NCBI Official Synonym Symbols: BCM1; BLAST; hCD48; mCD48; BLAST1; SLAMF2; MEM-102
• NCBI Protein Information: CD48 antigen
• UniProt Protein Name: CD48 antigen
• Protein Family: CD48 antigen
• UniProt Gene Name: CD48
• UniProt Synonym Gene Names: BCM1; BLAST1; SLAMF2

NCBI Description

This gene encodes a member of the CD2 subfamily of immunoglobulin-like receptors which includes SLAM (signaling lymphocyte activation molecules) proteins. The encoded protein is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation.