ERP27 recombinant protein

Recombinant Human ERP27 Protein (Fc tag)

Cat. Num. AAA2545347

• Gene Names: ERP27; PDIA8; C12orf46
• Purity: > 84 % as determined by SDS-PAGE
• Synonyms: ERP27; Recombinant Human ERP27 Protein (Fc tag); C12orf46 ERP27; ERP27 recombinant protein

• Host: Human Cells
• Purity/Purification: > 84 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 172

• Application Notes: The recombinant human ERP27/mFc is a disulfide-linked homodimer. The reduced monomer comprises 478 amino acids and has a predicted molecular mass of 53.7 kDa. The apparent molecular mass of the protein is approximately 59 in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Glu 26
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for ERP27 recombinant protein

Background: ERP27 contains 1 thioredoxin domain and is a noncatalytic member of the protein disulfide isomerase family. Protein disulfide isomerases (PDIs) constitute a family of structurally related enzymes which catalyze disulfide bonds formation, reduction, or isomerization of newly synthesized proteins in the lumen of the endoplasmic reticulum (ER). They act also as chaperones, and are, therefore, part of a quality-control system for the correct folding of the proteins in the same subcellular compartment. PDI has been found to have moderate effects (25-fold) on the rate of oxidative folding of proteins in vitro. Recombinant Human Protein Disulfide Isomerase is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. Recombinant PDI has been found to have moderate effects (25-fold) on the rate of oxidative folding of proteins in vitro. ERP27 is a widely expressed protein which localizes to the ER and may act as a protease, protein disulfide isomerase, thiol-disulfide oxidase or phospholipase. ERP27 doesn't contain a CXXC active site motif indicating that it is a catalytically redox-inactive member of the protein disulfide isomerase family.

Description: A DNA sequence encoding the human ERP27 (Q96DN0) (Glu26-Pro269) was fused with Fc region of mouse IgG at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 663854316
• NCBI GeneID: 121506
• NCBI Accession #: NP_001287713.1
• NCBI GenBank Nucleotide #: NM_001300784.1
• UniProt Accession #: Q96DN0
• Molecular Weight: 30,480 Da
• NCBI Official Full Name: endoplasmic reticulum resident protein 27 isoform 2
• NCBI Official Synonym Full Names: endoplasmic reticulum protein 27
• NCBI Official Symbol: ERP27
• NCBI Official Synonym Symbols: PDIA8; C12orf46
• NCBI Protein Information: endoplasmic reticulum resident protein 27
• UniProt Protein Name: Endoplasmic reticulum resident protein 27
• Protein Family: Endoplasmic reticulum resident protein
• UniProt Gene Name: ERP27
• UniProt Synonym Gene Names: C12orf46; ER protein 27; ERp27

NCBI Description

This gene encodes a noncatalytic member of the protein disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins. The canonical protein has an N-terminal signal sequence, two thioredoxin (TRX)-like domains and a C-terminal ER-retention sequence. Alternative splicing results in multiple transcript variants encoding distinct isoforms; some of which lack domains present in the canonical protein. [provided by RefSeq, Dec 2016]

Uniprot Description

ERP27: Belongs to the protein disulfide isomerase family. Interacts with PDIA3. Binds somatostatin-14 via hydrophobic interactions

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12p12.3

Cellular Component: endoplasmic reticulum

Molecular Function: protein binding; protein disulfide isomerase activity

Biological Process: protein folding