ERP27 recombinant protein

ERP27 Protein, Human, Recombinant (mFc Tag)

Cat. Num. AAA8121358

• Gene Names: ERP27; PDIA8; C12orf46
• Purity: >84% as determined by SDS-PAGE
• Synonyms: ERP27; ERP27 Protein; Human; Recombinant (mFc Tag); Human ERP27 Protein (Fc Tag); C12orf46 Protein; PDIA8 Protein; endoplasmic reticulum protein 27; ERP27 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >84% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Glu26-Pro269

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 26
• Tag: C-mouse IgG1-Fc
• Protein Construction: A DNA sequence encoding the human ERP27 (Q96DN0)(Glu26-Pro269) was fused with Fc region of mouse IgG at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ERP27 recombinant protein

Background: ERP27 contains 1 thioredoxin domain and is a noncatalytic member of the protein disulfide isomerase family. Protein disulfide isomerases (PDIs) constitute a family of structurally related enzymes which catalyze disulfide bonds formation, reduction, or isomerization of newly synthesized proteins in the lumen of the endoplasmic reticulum (ER). They act also as chaperones, and are, therefore, part of a quality-control system for the correct folding of the proteins in the same subcellular compartment. PDI has been found to have moderate effects (25-fold) on the rate of oxidative folding of proteins in vitro. Recombinant Human Protein Disulfide Isomerase is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. Recombinant PDI has been found to have moderate effects (25-fold) on the rate of oxidative folding of proteins in vitro. ERP27 is a widely expressed protein which localizes to the ER and may act as a protease, protein disulfide isomerase, thiol-disulfide oxidase or phospholipase. ERP27 doesn't contain a CXXC active site motif indicating that it is a catalytically redox-inactive member of the protein disulfide isomerase family.

References

Lamesch P, et al. (2007) hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes. Genomics. 89(3):307-15.Ota T, et al. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 36(1):40-5.Alanen HI, et al. (2006) ERp27, a new non-catalytic endoplasmic reticulum-located human protein disulfide isomerase family member, interacts with ERp57. J Biol Chem. 281(44):33727-38.

NCBI and Uniprot Product Information

• NCBI GI #: 663854316
• NCBI GeneID: 121506
• NCBI Accession #: NP_001287713.1
• NCBI GenBank Nucleotide #: NM_001300784.1
• UniProt Accession #: Q96DN0
• Molecular Weight: 30,480 Da
• NCBI Official Full Name: endoplasmic reticulum resident protein 27 isoform 2
• NCBI Official Synonym Full Names: endoplasmic reticulum protein 27
• NCBI Official Symbol: ERP27
• NCBI Official Synonym Symbols: PDIA8; C12orf46
• NCBI Protein Information: endoplasmic reticulum resident protein 27; ER protein 27; endoplasmic reticulum protein 27 kDa; protein disulfide isomerase family A, member 8
• UniProt Protein Name: Endoplasmic reticulum resident protein 27
• Protein Family: Endoplasmic reticulum resident protein
• UniProt Gene Name: ERP27
• UniProt Synonym Gene Names: C12orf46; ER protein 27; ERp27
• UniProt Entry Name: ERP27_HUMAN

NCBI Description

ERP27 is a noncatalytic member of the protein disulfide isomerase (PDI; see MIM 608012) family of endoplasmic reticulum (ER) proteins (Alanen et al., 2006 [PubMed 16940051]).[supplied by OMIM, Mar 2008]

Uniprot Description

ERP27: Belongs to the protein disulfide isomerase family. Interacts with PDIA3. Binds somatostatin-14 via hydrophobic interactions

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12p12.3

Cellular Component: cell surface; endoplasmic reticulum lumen; endoplasmic reticulum

Molecular Function: protein disulfide isomerase activity

Biological Process: protein folding