Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ERP27 antibody at 1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
Rabbit anti-Mouse, Rat ERP27 Polyclonal Antibody | anti-ERP27 antibody
ERP27 Rabbit pAb
Gene Names
ERP27; PDIA8; C12orf46
Reactivity
Mouse, Rat
Applications
Western Blot
Purity
Affinity purification
Synonyms
ERP27; Polyclonal Antibody; ERP27 Rabbit pAb; C12orf46; PDIA8; anti-ERP27 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Sequence
MEAAPSRFMFLLFLLTCELAAEVAAEVEKSSDGPGAAQEPTWLTDVPAAMEFIAATEVAVIGFFQDLEIPAVPILHSMVQKFPGVSFGISTDSEVLTHYNITGNTICLFRLVDNEQLNLEDEDIESIDATKLSRFIEINSLHMVTEYNPVTVIGLFNSVIQIHLLLIMNKASPEYEENMHRYQKAAKLFQGKILFILVDSGMKENGKVISFFKLKESQLPALAIYQTLDDEWDTLPTAEVSVEHVQNFCDGFLSGKLLKENRESEGKTPKVEL
Applicable Applications for anti-ERP27 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human ERP27 (NP_689534.1).
Cellular Location
Endoplasmic reticulum lumen
Positive Samples
Mouse pancreas, Mouse spleen, Rat lung
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ERP27 antibody at 1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ERP27 antibody at 1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
Related Product Information for anti-ERP27 antibody
Background: This gene encodes a noncatalytic member of the protein disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins. The canonical protein has an N-terminal signal sequence, two thioredoxin (TRX)-like domains and a C-terminal ER-retention sequence. Alternative splicing results in multiple transcript variants encoding distinct isoforms; some of which lack domains present in the canonical protein.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30,480 Da
NCBI Official Full Name
endoplasmic reticulum resident protein 27 isoform 2
NCBI Official Synonym Full Names
endoplasmic reticulum protein 27
NCBI Official Symbol
ERP27
NCBI Official Synonym Symbols
PDIA8; C12orf46
NCBI Protein Information
endoplasmic reticulum resident protein 27; ER protein 27; endoplasmic reticulum protein 27 kDa; protein disulfide isomerase family A, member 8
UniProt Protein Name
Endoplasmic reticulum resident protein 27
Protein Family
UniProt Gene Name
ERP27
UniProt Synonym Gene Names
C12orf46; ER protein 27; ERp27
UniProt Entry Name
ERP27_HUMAN
NCBI Description
ERP27 is a noncatalytic member of the protein disulfide isomerase (PDI; see MIM 608012) family of endoplasmic reticulum (ER) proteins (Alanen et al., 2006 [PubMed 16940051]).[supplied by OMIM, Mar 2008]
Uniprot Description
ERP27: Belongs to the protein disulfide isomerase family. Interacts with PDIA3. Binds somatostatin-14 via hydrophobic interactions
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 12p12.3
Cellular Component: cell surface; endoplasmic reticulum lumen; endoplasmic reticulum
Molecular Function: protein disulfide isomerase activity
Biological Process: protein folding
Research Articles on ERP27
Similar Products
Product Notes
The ERP27 erp27 (Catalog #AAA9142130) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ERP27 Rabbit pAb reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ERP27 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the ERP27 erp27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MEAAPSRFMF LLFLLTCELA AEVAAEVEKS SDGPGAAQEP TWLTDVPAAM EFIAATEVAV IGFFQDLEIP AVPILHSMVQ KFPGVSFGIS TDSEVLTHYN ITGNTICLFR LVDNEQLNLE DEDIESIDAT KLSRFIEINS LHMVTEYNPV TVIGLFNSVI QIHLLLIMNK ASPEYEENMH RYQKAAKLFQ GKILFILVDS GMKENGKVIS FFKLKESQLP ALAIYQTLDD EWDTLPTAEV SVEHVQNFCD GFLSGKLLKE NRESEGKTPK VEL. It is sometimes possible for the material contained within the vial of "ERP27, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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